TNN Polyklonaler Antikörper

TNN Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24839-1-AP

Synonyme

tenascin N, TN W, TN N, tenascin-N

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  WB analysis using 24839-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24839-1-AP (TNN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24839-1-AP bindet in WB, ELISA TNN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TNN fusion protein Ag18127
• Vollständiger Name: tenascin N
• Berechnetes Molekulargewicht: 1299 aa, 144 kDa
• Beobachtetes Molekulargewicht: 144 kDa, 173 kDa
• GenBank-Zugangsnummer: BC136619
• Gene symbol: TNN
• Gene ID (NCBI): 63923
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNN (Tenascin-W), also known as TNW. It is expected to be located in the extracellular space. The protein is not detected in normal adult mammary tissues or brain but expressed in most breast tumors and brain tumors, such as glioblastomas, astrocytomas and oligodendrogliomas, tested (PMID:17909022). And in brain tumors, detected around the endothelial cell layer of the clood vessels (PMID: 19884327). TNN is the fourth and final member of the extracellular matrix protein family of tenascins. In vitro, the presence of tenascin-W increased the proportion of elongated human umbilical vein endothelial cells (HUVECs) and augmented the mean speed of cell migration. Furthermore, tenascin-W triggered sprouting of HUVEC spheroids to a similar extent as the proangiogenic factor tenascin-C. Tenascin-W is a specific marker of glioma-associated blood vessels and stimulates angiogenesis in vitro(PMID: 19884327). The predicted molecular weight of the protein is 144 kDa in human and 173 kDa in mouse.