TNNI2 Polyklonaler Antikörper

TNNI2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 11634-1-AP

Synonyme

AMCD2B, DA2B, FSSV, fsTnI, TNNI2, Troponin I fast skeletal muscle

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  WB analysis using 11634-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 11634-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skeletal muscle using 11634-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 11634-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 11634-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 11634-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11634-1-AP (TNNI2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 11634-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 11634-1-AP (TNNI2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 11634-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 11634-1-AP (TNNI2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse skeletal muscle using 11634-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11634-1-AP (TNNI2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 11634-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11634-1-AP (TNNI2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, humanes Nierengewebe, humanes Skelettmuskelgewebe, humanes Milzgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

11634-1-AP bindet in WB, IHC, ELISA TNNI2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TNNI2 fusion protein Ag2208
• Vollständiger Name: troponin I type 2 (skeletal, fast)
• Berechnetes Molekulargewicht: 182 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC032148
• Gene symbol: TNNI2
• Gene ID (NCBI): 7136
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Heliyon; Exogenous hydrogen sulfide enhances myogenic differentiation of C2C12 myoblasts under high palmitate stress; Authors - Fangping Lu