TNNI2 Polyklonaler Antikörper

TNNI2 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 22130-1-AP

Synonyme

Troponin I fast skeletal muscle

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  WB analysis of mouse skeletal muscle using 22130-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22130-1-AP (TNNI2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22130-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22130-1-AP (TNNI2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 22130-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22130-1-AP (TNNI2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 22130-1-AP

  IP result of anti-TNNI2 (IP:22130-1-AP, 4ug; Detection:22130-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug.

• 

  IHC staining of human skeletal muscle using 22130-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22130-1-AP (TNNI2 Antibody) at dilution of 1:50.

• 

  IHC staining of mouse skeletal muscle using 22130-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22130-1-AP (TNNI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skeletal muscle using 22130-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TNNI2 antibody (22130-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 22130-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TNNI2 antibody (22130-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

22130-1-AP bindet in WB, IHC, IF-P, IP, ELISA TNNI2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TNNI2 fusion protein Ag17750
• Vollständiger Name: troponin I type 2 (skeletal, fast)
• Berechnetes Molekulargewicht: 182 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21-26 kDa
• GenBank-Zugangsnummer: BC032148
• Gene symbol: TNNI2
• Gene ID (NCBI): 7136
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Ann Surg Oncol; Troponin I2 as a Specific Biomarker for Prediction of Peritoneal Metastasis in Gastric Cancer.; Authors - Koichi Sawaki
mouse	WB	Sci Rep; A missense mutation in the RSRSP stretch of Rbm20 causes dilated cardiomyopathy and atrial fibrillation in mice.; Authors - Kensuke Ihara
mouse	WB	Front Physiol; Lipidomic Analyses Reveal Specific Alterations of Phosphatidylcholine in Dystrophic Mdx Muscle.; Authors - William J Valentine
mouse	WB	J Muscle Res Cell Motil; Differential regulation of Actn2 and Actn3 expression during unfolded protein response in C2C12 myotubes.; Authors - Nagakatsu Harada