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TNP1 Polyklonaler Antikörper

TNP1 Polyklonal Antikörper für WB, IF-P, IF-Fro, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF-P, IF-Fro, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17178-1-PBS

Synonyme

STP-1, TP-1

Filter:
  • Western Blot (WB) analysis of mouse testis tissue using TNP1 Polyclonal antibody (17178-1-AP)

    WB analysis of mouse testis using 17178-1-AP (same clone as 17178-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17178-1-AP (TNP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17178-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using TNP1 Polyclonal antibody (17178-1-AP)

    WB analysis of mouse testis using 17178-1-AP (same clone as 17178-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17178-1-AP (TNP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17178-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using TNP1 Polyclonal antibody (17178-1-AP)

    IF Staining of mouse testis using 17178-1-AP (same clone as 17178-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 17178-1-AP (TNP1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17178-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using TNP1 Polyclonal antibody (17178-1-AP)

    IF Staining of mouse testis using 17178-1-AP (same clone as 17178-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse testis tissue using 17178-1-AP (TNP1 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17178-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

17178-1-PBS bindet in WB, IF-P, IF-Fro, Indirect ELISA TNP1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TNP1 fusion protein Ag10890
Vollständiger Name transition protein 1 (during histone to protamine replacement)
Berechnetes Molekulargewicht 55 aa, 7 kDa
Beobachtetes Molekulargewicht 6-10 kDa
GenBank-ZugangsnummerBC029516
Gene symbol TNP1
Gene ID (NCBI) 7141
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transition nuclear proteins (TNP1 and TNP2) are the major nuclear proteins that replace somatic histones during spermatogenesis. TNPs are required for normal chromatin condensation and functional sperm development, spermatogenesis was found to be compromised in both Tnp1 and Tnp2 null mice. TNP1, or TP1, localized in nucleus, is a spermatid-specific product of the haploid genome which replaces histone and is itself replaced in the mature sperm by the protamines. Recently, TNP-1 was used as a germ cell marker in condensing spermatids.