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TNPO1 Rekombinanter Antikörper

TNPO1 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242269B5

Kat-Nr. : 85007-1-PBS

Synonyme

transportin 1, Transportin1, Transportin-1, 242269B5, Importin beta 2

Filter:
  • Western Blot (WB) analysis of various lysates using TNPO1 Recombinant antibody (85007-1-RR)

    WB analysis using 85007-1-RR (same clone as 85007-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85007-1-RR (TNPO1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85007-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TNPO1 Recombinant antibody (85007-1-RR)

    WB analysis of HeLa using 85007-1-RR (same clone as 85007-1-PBS)

    WB result of TNPO1 antibody (85007-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TNPO1 transfected HeLa cells. This data was developed using the same antibody clone with 85007-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

85007-1-PBS bindet in WB, Indirect ELISA TNPO1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name transportin 1
Berechnetes Molekulargewicht 102 kDa
Beobachtetes Molekulargewicht90 kDa
GenBank-ZugangsnummerNM_002270
Gene symbol Transportin-1
Gene ID (NCBI) 3842
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNPO1, also named as KPNB2, MIP1, TRN and MIP, belongs to the importin beta family. TNPO1 functions in nuclear protein import as nuclear transport receptor. It serves as receptor for nuclear localization signals (NLS) in cargo substrates. TNPO1 is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. TNPO1 is involved in nuclear import of M9-containing proteins. In vitro, it binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. It is involved in hnRNP A1/A2 nuclear export. TNPO1 mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. It binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, it mediates nuclear import of H2A, H2B, H3 and H4 histones, and SRP19. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. The antibody is specific to TNPO1.