TNPO2 Polyklonaler Antikörper

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• KD/KO Validated

TNPO2 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 17831-1-AP

Synonyme

TNPO 2, KPNB2B, Karyopherin beta-2b, IPO3, Importin 3

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  WB analysis of fetal human brain using 17831-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17831-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17831-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17831-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 17831-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 17831-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TNPO2 antibody (17831-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

17831-1-AP bindet in WB, IF/ICC, CoIP, ELISA TNPO2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TNPO2 fusion protein Ag12229
• Vollständiger Name: transportin 2
• Berechnetes Molekulargewicht: 887 aa, 100 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC072420
• Gene symbol: TNPO2
• Gene ID (NCBI): 30000
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Cell Rep; Hypertonic Stress Causes Cytoplasmic Translocation of Neuronal, but Not Astrocytic, FUS due to Impaired Transportin Function.; Authors - Eva-Maria Hock
human	WB,IF	Sci Rep; Transportin-2 plays a critical role in nucleocytoplasmic shuttling of oestrogen receptor-α.; Authors - Tetsuji Moriyama ; KD Validated
mouse	WB	eLife; Kap-β2/Transportin mediates β-catenin nuclear transport in Wnt signaling; Authors - Woong Y Hwang
human	WB,CoIP	Int J Biol Macromol; Membrane-bound transcription factor LRRC4 inhibits glioblastoma cell motility; Authors - Yang Liu
human	WB	J Biol Chem; The nuclear transportation of CHRONO regulates the circadian rhythm; Authors - Qin Zhou