Tenascin-R Polyklonaler Antikörper

Tenascin-R Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 19730-1-AP

Synonyme

Janusin, Restrictin, Tenascin R, TN R, TNR

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Galerie der Validierungsdaten

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  WB analysis of human brain using 19730-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19730-1-AP (Tenascin-R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 19730-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19730-1-AP (Tenascin-R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 19730-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19730-1-AP (Tenascin-R antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 19730-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19730-1-AP (Tenascin-R antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

19730-1-AP bindet in WB, IHC, ELISA Tenascin-R und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tenascin R (restrictin, janusin)
• Berechnetes Molekulargewicht: 150 kDa
• Beobachtetes Molekulargewicht: 180 kDa, 160 kDa
• GenBank-Zugangsnummer: NM_003285
• Gene symbol: TNR
• Gene ID (NCBI): 7143
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNR, also named as Restrictin and Janusin, belongs to the tenascin family. Neural extracellular matrix (ECM) protein involved in interactions with different cells and matrix components. These interactions can influence cellular behavior by either evoking a stable adhesion and differentiation, or repulsion and inhibition of neurite growth. Binding to cell surface gangliosides, TNR inhibits RGD-dependent integrin-mediated cell adhesion and results in an inhibition of PTK2 (FAK) phosphorylation and cell detachment. Binding to membrane surface sulfatides, TNR results in a oligodendrocyte adhesion and differentiation. Interaction with CNTN1, TNR induces a repulsion of neurons and an inhibition of neurite outgrowth. Interacts with SCN2B, TNR may play a crucial role in clustering and regulation of activity of sodium channels at nodes of Ranvier. TNR-linked chondroitin sulfate glycosaminoglycans are involved in the interaction with FN1 and mediate inhibition of cell adhesion and neurite outgrowth. The highly regulated addition of sulfated carbohydrate structure may modulate the adhesive properties of TNR over the course of development and during synapse maintenance. The antibody is specific to TNR.

Publikationen

Species	Application	Title
human	WB,IHC	Front Immunol; Immune Gene Signatures and Immunotypes in Immune Microenvironment Are Associated With Glioma Prognose.; Authors - Xiang-Xu Wang