TNS4 Polyklonaler Antikörper

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• KD/KO Validated

TNS4 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 11580-1-AP

Synonyme

Tensin-4, tensin 4, PP14434, C-terminal tensin-like protein, CTEN

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  WB analysis using 11580-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11580-1-AP (TNS4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 11580-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11580-1-AP (TNS4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 11580-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11580-1-AP (TNS4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 11580-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11580-1-AP (TNS4 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of A549 using 11580-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 11580-1-AP (TNS4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Plazenta-Gewebe, NIH/3T3-Zellen, PC-12-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

11580-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA TNS4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TNS4 fusion protein Ag2169
• Vollständiger Name: tensin 4
• Berechnetes Molekulargewicht: 715 aa, 77 kDa
• Beobachtetes Molekulargewicht: 77 kDa
• GenBank-Zugangsnummer: BC018706
• Gene symbol: TNS4
• Gene ID (NCBI): 84951
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNS4 (Tensin-4), also known as COOH-terminus tensin-like protein, is a distinctive member with the smallest molecular mass and lacks the N-terminal common region found in other family members. TNS4 plays a crucial role in CRC tumorigenesis, and that suppression of TNS4 would be an effective therapeutic strategy in treating a subset of cetuximab-refractory CRC patients including KRAS activating mutations (PMID: 31409052). TNS4 has been implicated in numerous vital biological processes, including proliferation, migration, cell adhesion, and apoptosis (PMID: 38164166).

Publikationen

Species	Application	Title
mouse	IF	Front Bioeng Biotechnol; Smarce1 and Tensin 4 Are Putative Modulators of Corneoscleral Stiffness.; Authors - Elizabeth M Boazak
human	WB,IHC	Oncol Lett; Tensin 4 facilitates aerobic glycolysis, migration and invasion of colorectal cancer cells through the β‑catenin/c‑Myc signaling pathway; Authors - Yan Wang ; KD Validated
human	WB	Pathol Res Pract; Tensin4 promotes invasion and migration of gastric cancer cells via regulating AKT/GSK-3β/snail signaling pathway.; Authors - Xiumin Qi ; KD Validated
human	IHC	Front Immunol; Development and functional validation of a disulfidoptosis-related gene prognostic model for lung adenocarcinoma based on bioinformatics and experimental validation; Authors - Tao Shen
human,mouse	WB,IP,IHC	Cancer Cell Int; TNS4 promotes lymph node metastasis of gastric cancer by interacting with integrin Β1 and inducing the activation of fibroblastic reticular cell; Authors - Xiang Zhang ; KD Validated
human	WB	Antioxid Redox Signal; A novel lncRNA FUAT1/TNS4 axis confers chemoresistance by suppressing ROS-mediated apoptosis in gastric cancer; Authors - Mingliang Liu