TOB1 Rekombinanter Antikörper

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• KD/KO Validated

TOB1 Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241043A5

Kat-Nr. : 83944-1-PBS

Synonyme

241043A5, APRO6, PIG49, Protein Tob1, TOB

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  WB analysis using 83944-1-RR (same clone as 83944-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83944-1-RR (TOB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83944-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 83944-1-RR (same clone as 83944-1-PBS)

  WB result of TOB1 antibody (83944-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOB1 transfected MCF-7 cells. This data was developed using the same antibody clone with 83944-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00930-3

  Cytometric bead array standard curve of MP00930-3, TOB1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83944-1-PBS. Detection antibody: 83944-2-PBS. Standard: Ag6714. Range: 0.313-20 ng/mL.

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  Cytometric bead array standard curve of MP00930-2

  Cytometric bead array standard curve of MP00930-2, TOB1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83944-1-PBS. Detection antibody: 83944-3-PBS. Standard: Ag6714. Range: 0.156-20 ng/mL.

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  Affinity and Kinetic Characterization of 83944-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83944-1-RR against Human TOB1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83944-1-PBS bindet in WB, Cytometric bead array, Indirect ELISA TOB1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TOB1 fusion protein Ag6714
• Vollständiger Name: transducer of ERBB2, 1
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 38-45 kDa
• GenBank-Zugangsnummer: BC031406
• Gene symbol: TOB1
• Gene ID (NCBI): 10140
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TOB1, also known as APRO6 or TROB1, is a member of the transducer of erbB-2/B-cell translocation gene protein family. Members of this family are anti-proliferative factors that have the potential to regulate cell growth. The TOB1 protein has a large additional C-terminal region, which contains PAM2 motifs that mediate interactions with poly(A)-binding proteins. It is mainly located in the cell nucleus and will transfer between the cell nucleus and the cytoplasm. The more phosphorylation, the more obvious the cytoplasmic localization.The predicted MW of this protein is 38 kDa. Catalog#83944-1-RR recognises 38 and 45 kDa band, and the additional 45 kDa band may be due to phosphorylation.