TOB1 Rekombinanter Antikörper

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• KD/KO Validated

TOB1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241043A5

Kat-Nr. : 83944-1-RR

Synonyme

241043A5, APRO6, PIG49, Protein Tob1, TOB

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  WB analysis of MCF-7 using 83944-1-RR

  WB result of TOB1 antibody (83944-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOB1 transfected MCF-7 cells.

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  WB analysis using 83944-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83944-1-RR (TOB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83944-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83944-1-RR against Human TOB1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T-Zellen, Jurkat-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83944-1-RR bindet in WB, ELISA TOB1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TOB1 fusion protein Ag6714
• Vollständiger Name: transducer of ERBB2, 1
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 38-45 kDa
• GenBank-Zugangsnummer: BC031406
• Gene symbol: TOB1
• Gene ID (NCBI): 10140
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TOB1, also known as APRO6 or TROB1, is a member of the transducer of erbB-2/B-cell translocation gene protein family. Members of this family are anti-proliferative factors that have the potential to regulate cell growth. The TOB1 protein has a large additional C-terminal region, which contains PAM2 motifs that mediate interactions with poly(A)-binding proteins. It is mainly located in the cell nucleus and will transfer between the cell nucleus and the cytoplasm. The more phosphorylation, the more obvious the cytoplasmic localization.The predicted MW of this protein is 38 kDa. Catalog#83944-1-RR recognises 38 and 45 kDa band, and the additional 45 kDa band may be due to phosphorylation.