TOB1 Rekombinanter Antikörper

TOB1 Rekombinant Antikörper für IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241043A9

Kat-Nr. : 83944-2-PBS

Synonyme

241043A9, APRO6, PIG49, Protein Tob1, TOB

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  IHC staining of human stomach cancer using 83944-2-RR (same clone as 83944-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83944-2-RR (TOB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83944-2-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 83944-2-RR (same clone as 83944-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83944-2-RR (TOB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83944-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83944-2-RR (same clone as 83944-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOB1 antibody (83944-2-RR, Clone: 241043A9 ) at dilution of 1:0 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83944-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00930-3

  Cytometric bead array standard curve of MP00930-3, TOB1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83944-1-PBS. Detection antibody: 83944-2-PBS. Standard: Ag6714. Range: 0.313-20 ng/mL.

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  Affinity and Kinetic Characterization of 83944-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83944-2-RR against Human TOB1 were performed. The affinity constant is 0.462 nM.

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Geprüfte Anwendungen

Produktinformation

83944-2-PBS bindet in IHC, IF/ICC, Cytometric bead array, Indirect ELISA TOB1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TOB1 fusion protein Ag6714
• Vollständiger Name: transducer of ERBB2, 1
• Berechnetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC031406
• Gene symbol: TOB1
• Gene ID (NCBI): 10140
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TOB1, also known as APRO6 or TROB1, is a member of the transducer of erbB-2/B-cell translocation gene protein family. Members of this family are anti-proliferative factors that have the potential to regulate cell growth. The TOB1 protein has a large additional C-terminal region, which contains PAM2 motifs that mediate interactions with poly(A)-binding proteins. It is mainly located in the cell nucleus and will transfer between the cell nucleus and the cytoplasm. The more phosphorylation, the more obvious the cytoplasmic localization.