TOE1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TOE1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (1)

Kat-Nr. : 16203-1-AP

Synonyme

Target of EGR1 protein 1, TOE1

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Galerie der Validierungsdaten

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  WB analysis using 16203-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16203-1-AP (TOE1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 16203-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16203-1-AP (TOE1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 16203-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16203-1-AP (TOE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16203-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16203-1-AP (TOE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16203-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16203-1-AP (TOE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16203-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16203-1-AP (TOE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16203-1-AP

  Immunofluorescent analysis of HepG2 cells, using TOE1 antibody 16203-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

16203-1-AP bindet in WB, IHC, IF, ELISA TOE1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TOE1 fusion protein Ag9172
• Vollständiger Name: target of EGR1, member 1 (nuclear)
• Berechnetes Molekulargewicht: 510 aa, 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC009364
• Gene symbol: TOE1
• Gene ID (NCBI): 114034
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TOE1, also known as hCaf1z, belongs to the DEDD family of deadenylases. TOE1 was initially identified as a direct target gene of ERG1, an immediate early transcription factor. TOE1 encodes an Asp-Glu-Asp-Asp deadenylase that specifically localizes to Cajal bodies in the nucleus, where assembly and maturation of small nuclear ribonucleoprotein particles, and telomerase ribonucleoprotein complex take place (PMID: 36257135). TOE1 has 2 isoforms with the molecular mass of 48 and 57 kDa.

Publikationen

Species	Application	Title
mouse	IF	Theriogenology; Knockdown of Toe1 causes developmental arrest during the morula-to-blastocyst transition in mice; Authors - Hongcheng Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Rhys (Verified Customer) (08-26-2019)

Superb antibody that works well for the detection for endogenous human TOE1 protein by western blotting.Lane 1: K562 wtGapLane 2: K562 control (empty vector)Lane 3: K562 TOE1 overexpressionGapLane 4: K562 scrambled shRNA controlLane 5: K562 TOE1 shRNA #1Lane 6: K562 TOE1 shRNA #2Lane 7: K562 TOE1 shRNA #3Lane 8: K562 TOE1 shRNA #4Lane 9: K562 TOE1 shRNA #5

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: K562 cells (Chronic Myeloid Leukaemia)

TOE1 Antibody Western Blot, validation (1/1000 dilution) in K562 cells (Chronic Myeloid Leukaemia) (Cat no:16203-1-AP)