TOM1L2 Polyklonaler Antikörper

TOM1L2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32179-1-PBS

Synonyme

Target Of Myb1 Like 2 Membrane Trafficking Protein, Target of Myb-like protein 2, TOM1-like protein 2

---

• 

  WB analysis using 32179-1-AP (same clone as 32179-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 32179-1-AP (TOM1L2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32179-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 32179-1-AP (same clone as 32179-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 32179-1-AP (TOM1L2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32179-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 32179-1-AP (same clone as 32179-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 32179-1-AP (TOM1L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32179-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 32179-1-AP (same clone as 32179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32179-1-AP (TOM1L2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32179-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 32179-1-AP (same clone as 32179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32179-1-AP (TOM1L2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32179-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 32179-1-AP (same clone as 32179-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TOM1L2 antibody (32179-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 32179-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

32179-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TOM1L2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TOM1L2 fusion protein Ag37612
• Vollständiger Name: target of myb1-like 2 (chicken)
• Berechnetes Molekulargewicht: 56 kDa，507aa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: NM_001082968.1
• Gene symbol: TOM1L2
• Gene ID (NCBI): 146691
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Target of Myb-like protein 2 (TOM1L2) acts as a MYO6/Myosin VI adapter protein that targets myosin VI to endocytic structures (PMID: 23023224). It may play a role in recruiting clathrin to endosomes and regulating growth factor-induced mitogenic signaling (PMID: 16412388; 16479011). TOM1L2 also regulates membrane trafficking that is linked to immunity and cell proliferation (PMID: 20604899).