TOM20 Monoklonaler Antikörper

TOM20 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

1D6F5

Kat-Nr. : 66777-1-PBS

Synonyme

TOMM20, TOMM 20, tom 20, MOM19, MAS20

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  WB analysis using 66777-1-Ig (same clone as 66777-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66777-1-Ig (TOM20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66777-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66777-1-Ig (same clone as 66777-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66777-1-Ig (TOM20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66777-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66777-1-Ig (same clone as 66777-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66777-1-Ig (TOM20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66777-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66777-1-Ig (same clone as 66777-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66777-1-Ig (TOM20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66777-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66777-1-Ig (same clone as 66777-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66777-1-Ig (TOM20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66777-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66777-1-Ig (same clone as 66777-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOM20 antibody (66777-1-Ig, Clone: 1D6F5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66777-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66777-1-Ig (same clone as 66777-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOM20 antibody (66777-1-Ig, Clone: 1D6F5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red). This data was developed using the same antibody clone with 66777-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66777-1-Ig (same clone as 66777-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TOM20 (66777-1-Ig, Clone:1D6F5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66777-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66777-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), ELISA TOM20 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TOM20 fusion protein Ag2378
• Vollständiger Name: translocase of outer mitochondrial membrane 20 homolog (yeast)
• Berechnetes Molekulargewicht: 145 aa, 16 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC000882
• Gene symbol: TOM20
• Gene ID (NCBI): 9804
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TOM20, also named as KIAA0016, belongs to the Tom20 family. It is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22, TOM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. TOM20 is characterized as major docking receptors to mediate the recognition by different mechanisms.