TOM20 Rekombinanter Antikörper

TOM20 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, Huhn, human, Maus, Ratte, Zebrafisch

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D22

Kat-Nr. : 80501-1-PBS

Synonyme

TOMM20, 1D22, KIAA0016, MAS20, MOM19

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  WB analysis using 80501-1-RR (same clone as 80501-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80501-1-RR (TOM20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80501-1-PBS in a different storage buffer formulation.

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  WB analysis of zebrafish using 80501-1-RR (same clone as 80501-1-PBS)

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 80501-1-RR (TOM20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80501-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 80501-1-RR (same clone as 80501-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80501-1-RR (TOM20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80501-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 80501-1-RR (same clone as 80501-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80501-1-RR (TOM20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80501-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 80501-1-RR (same clone as 80501-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOM20 antibody (80501-1-RR, Clone: 1D22 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80501-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 80501-1-RR (same clone as 80501-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOM20 antibody (80501-1-RR, Clone: 1D22 ) at dilution of 1:240 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80501-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 80501-1-RR (same clone as 80501-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using TOM20 antibody (80501-1-RR, Clone: 1D22 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 80501-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 80501-1-RR (same clone as 80501-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOM20 antibody (80501-1-RR, Clone: 1D22 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 80501-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80501-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80501-1-RR against Human TOM20 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

80501-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TOM20 und zeigt Reaktivität mit Hausschwein, Huhn, human, Maus, Ratten, Zebrafisch

• Getestete Reaktivität: Hausschwein, Huhn, human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TOM20 fusion protein Ag2378
• Vollständiger Name: translocase of outer mitochondrial membrane 20 homolog (yeast)
• Berechnetes Molekulargewicht: 145 aa, 16 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC000882
• Gene symbol: TOM20
• Gene ID (NCBI): 9804
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TOM20, also named as KIAA0016, belongs to the Tom20 family. It is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22, TOM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. TOM20 is characterized as major docking receptors to mediate the recognition by different mechanisms.