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TOM20 Rekombinanter Antikörper

TOM20 Rekombinant Antikörper für IF/ICC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, Huhn, human, Maus, Ratte, Zebrafisch

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

1D22

Kat-Nr. : CL555-80501

Synonyme

TOMM20, 1D22, KIAA0016, MAS20, MOM19

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 555-conjugated TOM20 Recombinant mo (CL555-80501)

    IF Staining of HepG2 using CL555-80501

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 555 TOM20 antibody (CL555-80501, Clone: 1D22 ) at dilution of 1:200.

Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL555-80501 bindet in IF/ICC TOM20 und zeigt Reaktivität mit Hausschwein, Huhn, human, Maus, Ratten, Zebrafisch

Getestete Reaktivität Hausschwein, Huhn, human, Maus, Ratte, Zebrafisch
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen TOM20 fusion protein Ag2378
Vollständiger Name translocase of outer mitochondrial membrane 20 homolog (yeast)
Berechnetes Molekulargewicht 145 aa, 16 kDa
Beobachtetes Molekulargewicht 16 kDa
GenBank-ZugangsnummerBC000882
Gene symbol TOM20
Gene ID (NCBI) 9804
Konjugation CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission maxima wavelengths554 nm / 570 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TOM20, also named as KIAA0016, belongs to the Tom20 family. It is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22, TOM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. TOM20 is characterized as major docking receptors to mediate the recognition by different mechanisms.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for CL Plus 555 TOM20 antibody CL555-80501Protokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen