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TOM70 Polyklonaler Antikörper

TOM70 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(94)

Kat-Nr. : 14528-1-AP

Synonyme

TOMM70A, KIAA0719, TOMM, TOMM70, Translocase of outer mitochondrial membrane protein 70



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMCF-7-Zellen, HeLa-Zellen, Maushirngewebe, Mausherzgewebe, Mauslebergewebe, Rattenherzgewebe, Ratten-Skelettmuskelgewebe
Erfolgreiche IPMaushirngewebe
Erfolgreiche Detektion in IHChumanes Herzgewebe, humanes Nierengewebe, humanes Lebergewebe, Mausherzgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen
Erfolgreiche Detektion in FC (Intra)HepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:6000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:20-1:200
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

14528-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA TOM70 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Affe, Maus, Ratte, Zebrafisch
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TOM70 fusion protein Ag6027
Vollständiger Name translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae)
Berechnetes Molekulargewicht 67 kDa
Beobachtetes Molekulargewicht 70 kDa
GenBank-ZugangsnummerBC052994
Gene symbol TOM70
Gene ID (NCBI) 9868
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TOMM70A (or TOM70) is a mitochondrial import receptor responsible for the import of cytosol synthesized precursor proteins into the mitochondria. Tom70 is a member of the tetratricopeptide repeat (TPR) co-chaperone family and contains a conserved TPR clamp domain for interaction with Hsc70 and Hsp90. Recently overexpression of TOM70 has been linked to chemoresistance to drugs in breast cancer cell lines. It is also believed that Tom70 is significantly associated with breast cancer relapse and may predict the recurrence of ovarian cancer.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for TOM70 antibody 14528-1-APProtokoll herunterladen
IHC protocol for TOM70 antibody 14528-1-APProtokoll herunterladenl
IF protocol for TOM70 antibody 14528-1-APProtokoll herunterladen
IP protocol for TOM70 antibody 14528-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Cell Res

Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression

Authors - Shiqiao Qiu
mouseWB

Nature

The ADP/ATP translocase drives mitophagy independent of nucleotide exchange.

Authors - Atsushi Hoshino
humanWB

Nature

In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer.

Authors - Milica Momcilovic
humanWB

Nature

Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.

Authors - Sarraf Shireen A SA
humanWB

Nature

The mitochondrial deubiquitinase USP30 opposes parkin-mediated mitophagy.

Authors - Baris Bingol
WB

Nat Med

The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity.

Authors - Wenzhang Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Kis (Verified Customer) (02-21-2025)

Nice antibody to stain mitochondria (1:300 for IF)

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: Human and rat liver cells
FH

Wenhui (Verified Customer) (01-02-2025)

This antibody works well us. It can give us a good signal in WB.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cardiac tissues and cardiomycoytes
FH

Wenhui (Verified Customer) (01-02-2025)

Antibody works for WB with cardiac tissues and cardiomyocytes

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: cardiac tissues and cardiomyoctes
FH

angela (Verified Customer) (12-27-2024)

very clear band

  • Applications: Western Blot
  • Primary Antibody Dilution: 1;1000
  • Cell Tissue Type: 293
TOM70 Antibody Western Blot validation (1;1000 dilution) in 293 (Cat no:14528-1-AP)
FH

Tom (Verified Customer) (04-15-2021)

Cells firxed with 3.7% paraformaldehyde. Antibody visualized using anti-rabbit Alexa Fluor 647 secondary.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Cos7
TOM70 Antibody Immunofluorescence validation (1:100 dilution) in Cos7 (Cat no:14528-1-AP)
FH

Daniela (Verified Customer) (09-10-2019)

Used for Western Blot on total cell lysate from HeLa, this antibody identifies a strong band slightly below 75 kDa (proteins run on 15% acrylamide gel) and several fainter no-specific bands.Used for co-immunoprecipitation to detect over-expressed Flag-tagged protein interacting with Tom70, it identifies a sharp single band at the expected MW.

  • Applications: Western Blot, Immunoprecipitation, Cell culture,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa cells