TOM70 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TOM70 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

65

Rezensionen (2)

Kat-Nr. : 14528-1-AP

Synonyme

TOM70, TOMM70A

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Galerie der Validierungsdaten

• 

  WB analysis of MCF-7 using 14528-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14528-1-AP (TOM70 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14528-1-AP

  WB result of TOM70 antibody (14528-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOM70 transfected HeLa cells.

• 

  WB analysis of rat heart using 14528-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 14528-1-AP (TOM70 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 14528-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14528-1-AP (TOM70 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14528-1-AP

  IP Result of anti-TOM70 (IP:14528-1-AP, 3ug; Detection:14528-1-AP 1:500) with mouse brain tissue lysate 6000ug.

• 

  IHC staining of human heart using 14528-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14528-1-AP (TOM70 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14528-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14528-1-AP (TOM70 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 14528-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14528-1-AP (TOM70 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 14528-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14528-1-AP (TOM70 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse heart using 14528-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14528-1-AP (TOM70 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 14528-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14528-1-AP (TOM70 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14528-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14528-1-AP (TOM70 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14528-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14528-1-AP (TOM70 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14528-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOM70 antibody (14528-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 14528-1-AP

  1X10^6 HeLa cells were stained with .2ug TOM70 antibody (14528-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HeLa-Zellen, Mausherzgewebe, Maushirngewebe, Mauslebergewebe, Rattenherzgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Lebergewebe, humanes Nierengewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 54 publications below
• IF: See 13 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

14528-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA TOM70 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TOM70 fusion protein Ag6027
• Vollständiger Name: translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 67 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC052994
• Gene symbol: TOMM70A
• Gene ID (NCBI): 9868
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TOMM70A (or TOM70) is a mitochondrial import receptor responsible for the import of cytosol synthesized precursor proteins into the mitochondria. Tom70 is a member of the tetratricopeptide repeat (TPR) co-chaperone family and contains a conserved TPR clamp domain for interaction with Hsc70 and Hsp90. Recently overexpression of TOM70 has been linked to chemoresistance to drugs in breast cancer cell lines. It is also believed that Tom70 is significantly associated with breast cancer relapse and may predict the recurrence of ovarian cancer.

Publikationen

Species	Application	Title
human	WB	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
mouse	WB	Nature; The ADP/ATP translocase drives mitophagy independent of nucleotide exchange.; Authors - Atsushi Hoshino
human	WB	Nature; In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer.; Authors - Milica Momcilovic
human	WB	Nature; Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.; Authors - Sarraf Shireen A SA
human	WB	Nature; The mitochondrial deubiquitinase USP30 opposes parkin-mediated mitophagy.; Authors - Baris Bingol
	WB	Nat Biotechnol; Efficient proximity labeling in living cells and organisms with TurboID.; Authors - Tess C Branon

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (04-15-2021)

Cells firxed with 3.7% paraformaldehyde. Antibody visualized using anti-rabbit Alexa Fluor 647 secondary.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Cos7

TOM70 Antibody Immunofluorescence validation (1:100 dilution) in Cos7 (Cat no:14528-1-AP)

FH

Daniela (Verified Customer) (09-10-2019)

Used for Western Blot on total cell lysate from HeLa, this antibody identifies a strong band slightly below 75 kDa (proteins run on 15% acrylamide gel) and several fainter no-specific bands.Used for co-immunoprecipitation to detect over-expressed Flag-tagged protein interacting with Tom70, it identifies a sharp single band at the expected MW.

• Applications: Western Blot, Immunoprecipitation, Cell culture,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cells

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