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  • KD/KO Validated

TOMM40 Polyklonaler Antikörper

TOMM40 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(112)

Kat-Nr. : 18409-1-AP

Synonyme

TOM40, C19orf1, D19S1177E, p38.5, PER EC1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Mausnierengewebe, Rattennierengewebe
Erfolgreiche IPMauslebergewebe
Erfolgreiche Detektion in IHChumanes Lebergewebe, Mausherzgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen, HeLa-Zellen
Erfolgreiche Detektion in FC (Intra)HepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

18409-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA TOMM40 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Affe, Maus, Ratte, Rind
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TOMM40 fusion protein Ag13065
Vollständiger Name translocase of outer mitochondrial membrane 40 homolog (yeast)
Berechnetes Molekulargewicht 38 kDa
Beobachtetes Molekulargewicht 38 kDa
GenBank-ZugangsnummerBC017224
Gene symbol TOMM40
Gene ID (NCBI) 10452
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The translocase of outer mitochondria membrane 40 (TOMM40, also known as TOM40), located in the center of the TOM complex, is a channel-forming subunit of translocase. It can facilitate the fluid movement of preproteins into the mitochondria by associating with TOMM20. TOMM40 plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by forming a complex with BCAP31 and mediating the translocation of Complex I components from the cytosol to the mitochondria (PMID: 31206022). TOMM40 has been reported to be associated with late-onset neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for TOMM40 antibody 18409-1-APProtokoll herunterladen
IHC protocol for TOMM40 antibody 18409-1-APProtokoll herunterladenl
IF protocol for TOMM40 antibody 18409-1-APProtokoll herunterladen
IP protocol for TOMM40 antibody 18409-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Nature

The ADP/ATP translocase drives mitophagy independent of nucleotide exchange.

Authors - Atsushi Hoshino
humanWB

Nature

In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer.

Authors - Milica Momcilovic
humanIF

Cell

Tau interactome maps synaptic and mitochondrial processes associated with neurodegeneration.

Authors - Tara E Tracy
humanWB

Cell Discov

The Fe-S cluster assembly protein IscU2 increases α-ketoglutarate catabolism and DNA 5mC to promote tumor growth

Authors - Xiaojun Ren
humanWB

Cell Metab

Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.

Authors - Marcel Morgenstern
  • KD Validated
monkeyWB,IF

Nat Cell Biol

MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.

Authors - Tim König

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Emilie (Verified Customer) (09-24-2025)

I applied it in IF (1:200), and it showed good performance.

  • Applications: Immunofluorescence
  • Cell Tissue Type: Fibroblasts
FH

Manon (Verified Customer) (09-17-2025)

We observed clear mitochondrial labeling with this antibody

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/200
  • Cell Tissue Type: Fibroblasts
FH

Yan (Verified Customer) (10-21-2024)

Very good protein signal in BJ-hTERT cells in IF. I would recommend it to assess mitochondrial morphology.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: BJ-hTERT
TOMM40 Antibody Immunofluorescence validation (1:1000 dilution) in BJ-hTERT (Cat no:18409-1-AP)
FH

Nuo (Verified Customer) (01-28-2023)

Good antibody for WB with high titer.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse cortex lysate
TOMM40 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:18409-1-AP)
FH

Süleyman (Verified Customer) (01-23-2022)

TOMM40 antibody (Green) is used 1:100 dilution (2 hour at RT) and 1:700 of Alexa Fluor 488 secondary antibody (1 hour RT). 4% Paraformaldehyde fixation for 10 min at RT. Permeabilization with 0.3% Triton X for 10-15 minutes at RT. 5% FBS in PBS used for blocking for 1 hour at RT. The image is colocalized with TOMM20 (Red). Blue is DAPI. As you can see there is colocalization with TOMM20 with TOMM40. It is a perfect colocalization. You might also reduce concentration to 1:300 as it was used low power of laser.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: HeLa FlipIn
TOMM40 Antibody Immunofluorescence validation (1:100 dilution) in HeLa FlipIn (Cat no:18409-1-AP)
FH

Tom (Verified Customer) (03-23-2021)

Cos7 cells fixed in 4% PFA and permeabilized in 0.1% Triton X-100. Cells blocked in 1% BSA in PBS.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Cos7
TOMM40 Antibody Immunofluorescence validation (1:200 dilution) in Cos7 (Cat no:18409-1-AP)
FH

Daniela (Verified Customer) (01-08-2020)

Immunoblot of total cell lysate of HeLa cell gives three bands, one specific for human Tom40 above 37 KDa on 15% acrylamide gel, the other two no specific and with much lower intensity. The anti-Tom40 antibody worked very well and specifically when conjugated with magnetic beads for immunoprecipitation.

  • Applications: Western Blot, Immunoprecipitation, Cell culture,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa cells