- Featured Product
- KD/KO Validated
TOMM40 Polyklonaler Antikörper
TOMM40 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (2)
Anwendung
WB, IP, IHC, IF, FC, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 18409-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Hirngewebe, Mausnierengewebe, Rattennierengewebe |
| Erfolgreiche IP | Mauslebergewebe |
| Erfolgreiche Detektion in IHC | humanes Lebergewebe, humanes Herzgewebe, humanes Nierengewebe, Mausherzgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF | HepG2-Zellen, HeLa-Zellen |
| Erfolgreiche Detektion in FC | HepG2-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:50-1:500 |
| Immunfluoreszenz (IF) | IF : 1:200-1:800 |
| Durchflusszytometrie (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 3 publications below |
| WB | See 66 publications below |
| IHC | See 1 publications below |
| IF | See 15 publications below |
| IP | See 1 publications below |
| CoIP | See 2 publications below |
Produktinformation
18409-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA TOMM40 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Affe, Maus, Ratte, Rind |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | TOMM40 fusion protein Ag13065 |
| Vollständiger Name | translocase of outer mitochondrial membrane 40 homolog (yeast) |
| Berechnetes Molekulargewicht | 38 kDa |
| Beobachtetes Molekulargewicht | 38 kDa |
| GenBank-Zugangsnummer | BC017224 |
| Gene symbol | TOMM40 |
| Gene ID (NCBI) | 10452 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
The translocase of outer mitochondria membrane 40 (TOMM40, also known as TOM40), located in the center of the TOM complex, is a channel-forming subunit of translocase. It can facilitate the fluid movement of preproteins into the mitochondria by associating with TOMM20. TOMM40 plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by forming a complex with BCAP31 and mediating the translocation of Complex I components from the cytosol to the mitochondria (PMID: 31206022). TOMM40 has been reported to be associated with late-onset neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease.
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for TOMM40 antibody 18409-1-AP | Protokoll herunterladen |
| IHC protocol for TOMM40 antibody 18409-1-AP | Protokoll herunterladen |
| IF protocol for TOMM40 antibody 18409-1-AP | Protokoll herunterladen |
| IP protocol for TOMM40 antibody 18409-1-AP | Protokoll herunterladen |
| FC protocol for TOMM40 antibody 18409-1-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Nature In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer. | ||
Cell Tau interactome maps synaptic and mitochondrial processes associated with neurodegeneration. | ||
Cell Discov The Fe-S cluster assembly protein IscU2 increases α-ketoglutarate catabolism and DNA 5mC to promote tumor growth | ||
Cell Metab Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
| ||
Nat Cell Biol MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Nuo (Verified Customer) (01-28-2023) | Good antibody for WB with high titer.
 |
FH Süleyman (Verified Customer) (01-23-2022) | TOMM40 antibody (Green) is used 1:100 dilution (2 hour at RT) and 1:700 of Alexa Fluor 488 secondary antibody (1 hour RT). 4% Paraformaldehyde fixation for 10 min at RT. Permeabilization with 0.3% Triton X for 10-15 minutes at RT. 5% FBS in PBS used for blocking for 1 hour at RT. The image is colocalized with TOMM20 (Red). Blue is DAPI. As you can see there is colocalization with TOMM20 with TOMM40. It is a perfect colocalization. You might also reduce concentration to 1:300 as it was used low power of laser.
 |
FH Tom (Verified Customer) (03-23-2021) | Cos7 cells fixed in 4% PFA and permeabilized in 0.1% Triton X-100. Cells blocked in 1% BSA in PBS.
 |
FH Daniela (Verified Customer) (01-08-2020) | Immunoblot of total cell lysate of HeLa cell gives three bands, one specific for human Tom40 above 37 KDa on 15% acrylamide gel, the other two no specific and with much lower intensity. The anti-Tom40 antibody worked very well and specifically when conjugated with magnetic beads for immunoprecipitation.
|
