TOMM40 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

TOMM40 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5E2C5

Kat-Nr. : 66658-1-PBS

Synonyme

TOM40, 5E2C5, C19orf1, D19S1177E, p38.5

---

• 

  WB analysis of HeLa using 66658-1-Ig (same clone as 66658-1-PBS)

  WB result of TOMM40 antibody (66658-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOMM40 transfected HeLa cells. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66658-1-Ig (same clone as 66658-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66658-1-Ig (same clone as 66658-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66658-1-Ig (same clone as 66658-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66658-1-Ig (same clone as 66658-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66658-1-Ig (same clone as 66658-1-PBS)

  pig brain tissue, rat brain tissue, and mouse brain tissue were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66658-1-Ig (same clone as 66658-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66658-1-Ig (same clone as 66658-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66658-1-Ig (TOMM40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66658-1-Ig (same clone as 66658-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66658-1-Ig (TOMM40 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66658-1-Ig (same clone as 66658-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66658-1-Ig (TOMM40 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66658-1-Ig (same clone as 66658-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66658-1-Ig (TOMM40 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

66658-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TOMM40 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TOMM40 fusion protein Ag13065
• Vollständiger Name: translocase of outer mitochondrial membrane 40 homolog (yeast)
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC017224
• Gene symbol: TOMM40
• Gene ID (NCBI): 10452
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.