TOMM40 Rekombinanter Antikörper

TOMM40 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250010E12

Kat-Nr. : 85773-4-PBS

Synonyme

TOM40, C19orf1, D19S1177E, p38.5, PER EC1

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  WB analysis using 85773-4-RR (same clone as 85773-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85773-4-RR (TOMM40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85773-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 85773-4-RR (same clone as 85773-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85773-4-RR (TOMM40 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85773-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 85773-4-RR (same clone as 85773-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85773-4-RR (TOMM40 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85773-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 85773-4-RR (same clone as 85773-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOMM40 antibody (85773-4-RR, Clone: 250010E12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85773-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85773-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85773-4-RR against Human TOMM40 were performed. The affinity constant is 1.59 nM.

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Geprüfte Anwendungen

Produktinformation

85773-4-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TOMM40 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TOMM40 fusion protein Ag13065
• Vollständiger Name: translocase of outer mitochondrial membrane 40 homolog (yeast)
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC017224
• Gene symbol: TOMM40
• Gene ID (NCBI): 10452
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The translocase of outer mitochondria membrane 40 (TOMM40, also known as TOM40), located in the center of the TOM complex, is a channel-forming subunit of translocase. It can facilitate the fluid movement of preproteins into the mitochondria by associating with TOMM20. TOMM40 plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by forming a complex with BCAP31 and mediating the translocation of Complex I components from the cytosol to the mitochondria (PMID: 31206022). TOMM40 has been reported to be associated with late-onset neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease.