TOP2A Monoklonaler Antikörper

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TOP2A Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E12B3

Kat-Nr. : 66541-1-PBS

Synonyme

DNA topoisomerase 2 alpha, TOP2, TOP2A, TP2A

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  WB analysis using 66541-1-Ig (same clone as 66541-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 66541-1-Ig (TOP2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66541-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66541-1-Ig (same clone as 66541-1-PBS)

  WB result of TOP2A antibody (66541-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2A transfected HepG2 cells. This data was developed using the same antibody clone with 66541-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66541-1-Ig (same clone as 66541-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66541-1-Ig (TOP2A antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66541-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66541-1-Ig (same clone as 66541-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66541-1-Ig (TOP2A antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66541-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66541-1-Ig (same clone as 66541-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66541-1-Ig (TOP2A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66541-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66541-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TOP2A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TOP2A fusion protein Ag19551
• Vollständiger Name: topoisomerase (DNA) II alpha 170kDa
• Berechnetes Molekulargewicht: 1531 aa, 174 kDa
• Beobachtetes Molekulargewicht: 174 kDa
• GenBank-Zugangsnummer: BC140791
• Gene symbol: TOP2A
• Gene ID (NCBI): 7153
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.