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TOP2B Rekombinanter Antikörper

TOP2B Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240949F10

Kat-Nr. : 84049-1-PBS

Synonyme

240949F10, DNA topoisomerase 2-beta, EC:5.6.2.2, top2beta, TOPIIB

Filter:
  • Western Blot (WB) analysis of various lysates using TOP2B Recombinant antibody (84049-1-RR)

    WB analysis using 84049-1-RR (same clone as 84049-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84049-1-RR (TOP2B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84049-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using TOP2B Recombinant antibody (84049-1-RR)

    WB analysis of HepG2 using 84049-1-RR (same clone as 84049-1-PBS)

    WB result of TOP2B antibody (84049-1-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2B transfected HepG2 cells. This data was developed using the same antibody clone with 84049-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TOP2B Recombinant antibody (84049-1-RR)

    IF Staining of HeLa using 84049-1-RR (same clone as 84049-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOP2B antibody (84049-1-RR, Clone: 240949F10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84049-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

84049-1-PBS bindet in WB, IF/ICC, ELISA TOP2B und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name topoisomerase (DNA) II beta 180kDa
Berechnetes Molekulargewicht 183 kDa
Beobachtetes Molekulargewicht180-190 kDa
GenBank-ZugangsnummerNM_001068
Gene symbol TOP2B
Gene ID (NCBI) 7155
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TOP2B belongs to the type II topoisomerase family. TOP2B control topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2B makes double-strand breaks. It is indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.