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TOPBP1 Polyklonaler Antikörper

TOPBP1 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23340-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of HeLa cells using TOPBP1 Polyclonal antibody (23340-1-AP)

    WB analysis of HeLa using 23340-1-AP (same clone as 23340-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 23340-1-AP (TOPBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23340-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TOPBP1 Polyclonal antibody (23340-1-AP)

    WB analysis of HeLa using 23340-1-AP (same clone as 23340-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 23340-1-AP (TOPBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23340-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TOPBP1 Polyclonal antibody (23340-1-AP)

    WB analysis of HeLa using 23340-1-AP (same clone as 23340-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 23340-1-AP (TOPBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23340-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

23340-1-PBS bindet in WB, Indirect ELISA TOPBP1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TOPBP1 fusion protein Ag18961
Vollständiger Name topoisomerase (DNA) II binding protein 1
Berechnetes Molekulargewicht 1522 aa, 171 kDa
Beobachtetes Molekulargewicht 171 kDa
GenBank-ZugangsnummerBC126209
Gene symbol TOPBP1
Gene ID (NCBI) 11073
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-affinitätsgereinigt
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human DNA topoisomerase II binding protein 1 (TopBP1) contains eight BRCT motifs that are found in proteins regulating the DNA damage response, transcription, and replication (1). In addition, TopBP1 shares sequence similarity with the fission yeast Rad4/Cut5 protein and the budding yeast DPB11 protein, both of which are required for DNA damage control and/or replication checkpoint control (2,3). Phosphorylation of TopBP1 occurs in response to DNA double-strand breaks and replication blocks (2). TopBP1 forms nuclear foci and localizes to the sites of DNA damage or the arrested replication forks (2,3). Downregulation of TopBP1 leads to reduced cell survival, probably due to increased apoptosis (2). TopBP1 functions as a transcriptional coactivator by enhancing the human papillomavirus (HPV) transcription/replication factor E2 (1). In addition, the HECT-domain ubiquitin ligase, hHYD, cooperates with TopBP1 in DNA damage response (4). TopBP1 specifically interacts with the C-terminal region of topoisomerase II beta, which suggests a supportive role for TopBP1 in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands (5). The gene encoding TopBP1 maps to chromosome 3q22.2 (5).