TORC2 Rekombinanter Antikörper

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• KD/KO Validated

TORC2 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242808B6

Kat-Nr. : 85150-4-RR

Synonyme

CRTC2, CRTC2,TORC2, 242808B6, CREB-regulated transcription coactivator 2, TORC 2

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  WB analysis of HepG2 using 85150-4-RR

  WB result of TORC2 antibody (85150-4-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TORC2 transfected HepG2 cells.

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  WB analysis using 85150-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85150-4-RR (CRTC2,TORC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 85150-4-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug TORC2 Recombinant antibody (85150-4-RR, Clone:242808B6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 85150-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85150-4-RR against Human TORC2 were performed. The affinity constant is 0.422 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, Raji-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85150-4-RR bindet in WB, FC (Intra), ELISA TORC2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TORC2 fusion protein Ag3167
• Vollständiger Name: CREB regulated transcription coactivator 2
• Berechnetes Molekulargewicht: 693 aa, 73 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC053562
• Gene symbol: TORC2
• Gene ID (NCBI): 200186
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CRTC2, also named as TORC2, belongs to the TORC family. It is a transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. It acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. CRTC2 enhances the interaction of CREB1 with TAF4. Ir regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. CRTC2 regulates the expression of specific genes such as the steroidogenic gene, StAR. TORC2 was recently shown to be an important regulator of gluconeogenesis in the livers of mammals. It is one of the other key regulators of CRE-dependent MIE gene expression in NT2 cells. This regulation is linked to VIP-induced TORC2 dephosphorylation and translocation to the nucleus. (PMID: 19369332, 20504934 ) .