TOX2 Rekombinanter Antikörper

TOX2 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241645C11

Kat-Nr. : 84304-5-RR

Synonyme

GCX-1, GCX1, C20orf100, 241645C11

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  WB analysis using 84304-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84304-5-RR (TOX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of A549 using 84304-5-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug TOX2 Recombinant antibody (84304-5-RR, Clone:241645C11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  FC experiment of MCF-7 using 84304-5-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug TOX2 Recombinant antibody (84304-5-RR, Clone:241645C11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 84304-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84304-5-RR against Human TOX2 were performed. The affinity constant is 110 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in FC (Intra): A549-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84304-5-RR bindet in WB, FC (Intra), ELISA TOX2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TOX2 fusion protein Ag14772
• Vollständiger Name: TOX high mobility group box family member 2
• Berechnetes Molekulargewicht: 488 aa, 52 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC007636
• Gene symbol: TOX2
• Gene ID (NCBI): 84969
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TOX2 is a transcription factor belonging to the TOX (thymocyte selection-associated HMG box) family that shares a highly conserved high mobility group DNA-binding domain with the other TOX members. A previous study showed that TOX2 is preferentially expressed in human NK cells among various leukocyte populations and is required for in vitro and in vivo human NK cell differentiation from UCB-derived CD341 hematopoietic stem cells (HSCs) (PMID: 25352127).