P53 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

P53 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(61)

Rezensionen (1)

Kat-Nr. : 21891-1-AP

Synonyme

TP53, Tumour Protein p53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13

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  WB analysis of A431 cells using 21891-1-AP

  WB result of p53 antibody (21891-1-AP, 1:1000) with sh-control and sh-p53 transfected A431 cells.

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  WB analysis of A431 using 21891-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21891-1-AP (TP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21891-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21891-1-AP (TP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 21891-1-AP

  IP result of anti-P53 (IP:21891-1-AP, 3ug; Detection:21891-1-AP 1:1000) with A431 cells lysate 1650ug.

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  IHC staining of human prostate cancer using 21891-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21891-1-AP (TP53 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 21891-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21891-1-AP (P53 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 21891-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21891-1-AP (P53 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 21891-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21891-1-AP (P53 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 21891-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 21891-1-AP (TP53 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 21891-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 21891-1-AP (TP53 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 21891-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21891-1-AP (TP53 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 21891-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 21891-1-AP (TP53 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary tumor using 21891-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 21891-1-AP (TP53 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human endometrial cancer using 21891-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 21891-1-AP (TP53 antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human endometrial cancer using 21891-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 21891-1-AP (TP53 antibody) at dilution of 1:400 (under 40x lens).

• 

  IF Staining of HepG2 using 21891-1-AP

  Immunofluorescent analysis of HepG2 cells using 21891-1-AP (TP53 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Endometriumkarzinomgewebe, humanes Gliomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 49 publications below
• IHC: See 19 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 3 publications below

Produktinformation

21891-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA P53 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: P53 fusion protein Ag16596
• Vollständiger Name: tumor protein p53
• Berechnetes Molekulargewicht: 393 aa, 44 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC003596
• Gene symbol: P53
• Gene ID (NCBI): 7157
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

1.       What is p53?

P53 is a tumor suppressor gene that plays a role in maintaining genomic stability and controlling apoptosis. During the cell cycle, it can arrest cells at the G1/S checkpoint and activate DNA repair mechanisms. It is the most mutated gene in cancer. In unstressed cells, p53 usually exists at low levels in an inactive form, being bound to Mdm2.

2.       FAQs and p53

a.       I fail to detect p53 by western blotting

Basal levels of wild-type p53 in untreated cells can be low. Try to load more cell lysate and use a positive control - a lysate of cells treated with DNA-damaging agents should increase p53 levels.

b.      I fail to detect p53 in some cell lines by western blotting

Various p53 mutations are present in cancer cell types. If mutations cause truncations/deletions some monoclonal antibodies may no longer recognize mutated p53. You have more chances of detecting various p53 mutants with our polyclonal antibody.

c.       I can detect more than one band ~50 kDa size / different cell lines give bands at slightly different size

p53 is a subject of post-translational modifications (http://p53.free.fr/p53_info/p53_modifications.html) and more than one isoform may be expressed (http://p53.free.fr/p53_info/p53_isoforms.html). Also, it is possible that your cell line of interest expresses one allele with mutated p53 with altered molecular weight.

Publikationen

Species	Application	Title
human	WB	Cancer Cell; p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.; Authors - Alessandro Carugo ; KD Validated
mouse	WB	Acta Pharmacol Sin; The PIWI-interacting RNA CRAPIR alleviates myocardial ischemia‒reperfusion injury by reducing p53-mediated apoptosis via binding to SRSF1; Authors - Hong Yan
	WB	J Control Release; Peritoneal M2 macrophage-derived extracellular vesicles as natural multitarget nanotherapeutics to attenuate cytokine storms after severe infections.; Authors - Yizhuo Wang
human	WB,IHC,CoIP	Cancer Lett; Choline-induced SLC5A7 impairs colorectal cancer growth by stabilizing p53 protein.; Authors - Yuan Yin
mouse	WB	Oxid Med Cell Longev; PIN1 Protects Hair Cells and Auditory HEI-OC1 Cells against Senescence by Inhibiting the PI3K/Akt/mTOR Pathway.; Authors - Yanzhuo Zhang
human,mouse	WB,IHC	Mol Ther Nucleic Acids; LINC00460-miR-149-5p/miR-150-5p-Mutant p53 Feedback Loop Promotes Oxaliplatin Resistance in Colorectal Cancer.; Authors - Xiangfeng Meng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Y (Verified Customer) (04-01-2019)

We really like the trial size, it lets us try to test if the antibody works on our experiment.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 200