TP53BP1-Specific Polyklonaler Antikörper

TP53BP1-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20002-1-PBS

Synonyme

TP53BP1, 53BP1, FLJ41424, p202, p53 binding protein 1

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  WB analysis using 20002-1-AP (same clone as 20002-1-PBS)

  HeLa lysates were subjected to SDS PAGE followed by western blot with 20002-1-AP (TP53BP1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20002-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20002-1-AP (same clone as 20002-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20002-1-AP (TP53BP1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20002-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 20002-1-AP (same clone as 20002-1-PBS)

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 20002-1-AP (TP53BP1-Specific antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20002-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 20002-1-AP (same clone as 20002-1-PBS)

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 20002-1-AP (TP53BP1-Specific antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20002-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 20002-1-AP (same clone as 20002-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TP53BP1-Specific antibody (20002-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20002-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20002-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TP53BP1-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tumor protein p53 binding protein 1
• Berechnetes Molekulargewicht: 214 kDa
• Beobachtetes Molekulargewicht: 450 kDa
• GenBank-Zugangsnummer: NM_005657
• Gene symbol: TP53BP1
• Gene ID (NCBI): 7158
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TP53BP1, also named as p53BP1, p202, 53BP1 and FLJ41424, may have a role in checkpoint signaling during mitosis. TP53BP1 enhances TP53-mediated transcriptional activation. TP53BP1 plays a role in the response to DNA damage. This antibody is specific to TP53BP1.