PIG3 Polyklonaler Antikörper

PIG3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 14828-1-AP

Synonyme

TP53I3, Quinone oxidoreductase PIG3, p53-induced gene 3 protein, p53 induced gene 3 protein, NADPH:quinone reductase PIG3

---

• 

  WB analysis of HeLa using 14828-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14828-1-AP (PIG3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14828-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14828-1-AP (PIG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14828-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14828-1-AP (PIG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14828-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14828-1-AP (PIG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 14828-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 14828-1-AP (PIG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 14828-1-AP

  IP result of anti-PIG3 (IP:14828-1-AP, 4ug; Detection:14828-1-AP 1:500) with MCF-7 cells lysate 3200ug.

• 

  IHC staining of human liver cancer using 14828-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14828-1-AP (PIG3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14828-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14828-1-AP (PIG3 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14828-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PIG3 antibody (14828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen, SMMC-7721-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

14828-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PIG3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIG3 fusion protein Ag6618
• Vollständiger Name: tumor protein p53 inducible protein 3
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 34-36 kDa
• GenBank-Zugangsnummer: BC000474
• Gene symbol: TP53I3
• Gene ID (NCBI): 9540
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IF	Biomaterials; Furin-instructed aggregated gold nanoparticles for re-educating tumor associated macrophages and overcoming breast cancer chemoresistance.; Authors - Rou Xie
human	WB	Virology; VP5 protein of oncolytic herpes simplex virus type 2 induces apoptosis in A549 cells through TP53I3 protein; Authors - Yang Wang
human	WB	Oncol Lett; Prognostic prediction signature and molecular subtype for liver cancer: A CTC/CTM‑related gene prediction model and independent external validation; Authors - Ling Xu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

IGNACIO (Verified Customer) (10-22-2021)

The antibody works perfect for WB with strong bands. The size is correct. Highly recommended.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: U2OS cancer cell lines