p63 Monoklonaler Antikörper

p63 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4D6G12

Kat-Nr. : 60332-2-PBS

Synonyme

TP63, tumor protein p63, Tumor protein 63, Transformation-related protein 63, TP 63

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  WB analysis of HaCaT using 60332-2-Ig (same clone as 60332-2-PBS)

  HaCaT cells were subjected to SDS PAGE followed by western blot with 60332-2-Ig (TP63 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 60332-2-Ig (same clone as 60332-2-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 60332-2-Ig (TP63 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 60332-2-Ig (same clone as 60332-2-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 60332-2-Ig (TP63 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

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  IHC staining of rat skin using 60332-2-Ig (same clone as 60332-2-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60332-2-Ig (same clone as 60332-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 60332-2-Ig (same clone as 60332-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 60332-2-Ig (same clone as 60332-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60332-2-Ig (same clone as 60332-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60332-2-Ig (same clone as 60332-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60332-2-Ig (TP63 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60332-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60332-2-PBS bindet in WB, IHC, Indirect ELISA p63 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: p63 fusion protein Ag2791
• Vollständiger Name: tumor protein p63
• Berechnetes Molekulargewicht: 680 aa, 77 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC039815
• Gene symbol: TP63
• Gene ID (NCBI): 8626
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TP63, also named KET, P63, P73H, P73L and TP73L, belongs to the p53 family. It is a homologue of the tumor suppressor p53 and p73 genes. It is involved in malignancy acquisition and maintenance of cells. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis. TP63 acts as a sequence specific DNA binding transcriptional activator or repressor. TP63 has 12 isoforms with MW 40kd(P40), 50kd(P60),63kd(P63) and 73kd(P73L). It is Nuclear stain.