TP63 Rekombinanter Antikörper

TP63 Rekombinant Antikörper für WB, IHC, ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

250042G4

Kat-Nr. : 86154-1-RR

Synonyme

p63, CUSP, TP 63, Transformation-related protein 63, Tumor protein 63

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  WB analysis using 86154-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86154-1-RR (TP63 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 86154-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86154-1-RR (TP63 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  ChIP experiment of HaCaT using 86154-1-RR

  Chromatin was prepared from HaCaT cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of TP63 (86154-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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  Affinity and Kinetic Characterization of 86154-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86154-1-RR against Human TP63 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HaCaT-Zellen, Maushautgewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in ChIP-qPCR: HaCaT-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• CHImmunpräzipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86154-1-RR bindet in WB, IHC, ELISA, ChIP-qPCR TP63 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TP63 fusion protein Ag2791
• Vollständiger Name: tumor protein p63
• Berechnetes Molekulargewicht: 680 aa, 77 kDa
• Beobachtetes Molekulargewicht: 63-68 kDa
• GenBank-Zugangsnummer: BC039815
• Gene symbol: TP63
• Gene ID (NCBI): 8626
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TP63, also named KET, P63, P73H, P73L and TP73L, belongs to the p53 family. It is a homologue of the tumor suppressor p53 and p73 genes. It is involved in malignancy acquisition and maintenance of cells. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis. TP63 acts as a sequence specific DNA binding transcriptional activator or repressor. TP63 has 12 isoforms with MW 40kd(P40), 50kd(P60),63kd(P63) and 73kd(P73L).