hD53; TPD52L1 Polyklonaler Antikörper

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• KD/KO Validated

hD53; TPD52L1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 14732-1-AP

Synonyme

D53, hD53, hD53; TPD52L1, TDP53, TPD52L1, TPD52L2, TPD53, tumor protein D52 like 1, Tumor protein D53

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 14732-1-AP

  WB result of hD53; TPD52L1 antibody (14732-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-hD53; TPD52L1 transfected MCF-7 cells.

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  WB analysis of MCF-7 using 14732-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14732-1-AP (hD53; TPD52L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 14732-1-AP

  IP Result of anti-hD53; TPD52L1 (IP:14732-1-AP, 3ug; Detection:14732-1-AP 1:300) with MCF-7 cells lysate 2500ug.

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  IHC staining of human prostate cancer using 14732-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14732-1-AP (hD53; TPD52L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 14732-1-AP

  Immunofluorescent analysis of MCF-7 cells using 14732-1-AP (hD53; TPD52L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, MCF-7-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Produktinformation

14732-1-AP bindet in WB, IP, IHC, IF, ELISA hD53; TPD52L1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: hD53; TPD52L1 fusion protein Ag6493
• Vollständiger Name: tumor protein D52-like 1
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC002375
• Gene symbol: TPD52L1
• Gene ID (NCBI): 7164
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tumor protein D52-like 1 (TPD52L1) gene, also known as hD53, encodes a member of the tumor protein D52 (TPD52) family. TPD52-like proteins are coiled-coil motif-bearing proteins first identified through their expression in human breast carcinoma, which have been proposed to represent signaling intermediates and regulators of vesicle trafficking. hD53 may form homo- or heterodimers with other TPD52 family members, and it is reported to be involved in cell proliferation and calcium signaling. Four variants resulted from alternative splicing have been predicated and this antibody is expected to recognize all the variants.

Publikationen

Species	Application	Title
human	WB	Cell Biosci; Tumor protein D52 is upregulated in oral squamous carcinoma cells under hypoxia in a hypoxia-inducible-factor-independent manner and is involved in cell death resistance.; Authors - Yuzo Abe
human	IHC	Front Oncol; Transcriptomic Analyses of the Adenoma-Carcinoma Sequence Identify Hallmarks Associated With the Onset of Colorectal Cancer.; Authors - Qin Hong
human,mouse	WB,IHC,IP	Int J Oncol; Opposite effects of tumor protein D (TPD) 52 and TPD54 on oral squamous cell carcinoma cells.; Authors - Kosuke Kato
	WB	Biochem J; Tumor protein D52 expression is post-transcriptionally regulated by T-cell intercellular antigen (TIA) 1 and TIA-related protein via mRNA stability.; Authors - Hiromi Motohashi
		Sci Data; The Signaling Pathways Project, an integrated 'omics knowledgebase for mammalian cellular signaling pathways.; Authors - Scott A Ochsner