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hD53; TPD52L1 Polyklonaler Antikörper

hD53; TPD52L1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 14732-1-AP

Synonyme

TPD53, TPD52L1, Tumor protein D52-like 1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMCF7 cells, MCF-7-Zellen
Erfolgreiche IPMCF-7-Zellen
Erfolgreiche Detektion in IHChumanes Prostatakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCMCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

14732-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA hD53; TPD52L1 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen hD53; TPD52L1 fusion protein Ag6493
Vollständiger Name tumor protein D52-like 1
Berechnetes Molekulargewicht 22 kDa
Beobachtetes Molekulargewicht 25 kDa
GenBank-ZugangsnummerBC002375
Gene symbol TPD52L1
Gene ID (NCBI) 7164
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tumor protein D52-like 1 (TPD52L1) gene, also known as hD53, encodes a member of the tumor protein D52 (TPD52) family. TPD52-like proteins are coiled-coil motif-bearing proteins first identified through their expression in human breast carcinoma, which have been proposed to represent signaling intermediates and regulators of vesicle trafficking. hD53 may form homo- or heterodimers with other TPD52 family members, and it is reported to be involved in cell proliferation and calcium signaling. Four variants resulted from alternative splicing have been predicated and this antibody is expected to recognize all the variants.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for hD53; TPD52L1 antibody 14732-1-APProtokoll herunterladen
IHC protocol for hD53; TPD52L1 antibody 14732-1-APProtokoll herunterladenl
IF protocol for hD53; TPD52L1 antibody 14732-1-APProtokoll herunterladen
IP protocol for hD53; TPD52L1 antibody 14732-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Cell Biosci

Tumor protein D52 is upregulated in oral squamous carcinoma cells under hypoxia in a hypoxia-inducible-factor-independent manner and is involved in cell death resistance.

Authors - Yuzo Abe
humanIHC

Front Oncol

Transcriptomic Analyses of the Adenoma-Carcinoma Sequence Identify Hallmarks Associated With the Onset of Colorectal Cancer.

Authors - Qin Hong
human,mouseWB,IHC,IP

Int J Oncol

Opposite effects of tumor protein D (TPD) 52 and TPD54 on oral squamous cell carcinoma cells.

Authors - Kosuke Kato
WB

Biochem J

Tumor protein D52 expression is post-transcriptionally regulated by T-cell intercellular antigen (TIA) 1 and TIA-related protein via mRNA stability.

Authors - Hiromi Motohashi

Sci Data

The Signaling Pathways Project, an integrated 'omics knowledgebase for mammalian cellular signaling pathways.

Authors - Scott A Ochsner
humanWB,IHC

Biomedicines

Tumor Protein D53 (TPD53): Involvement in Malignant Transformation of Low-Malignant Oral Squamous Cell Carcinoma Cells

Authors - Masataka Watanabe