TPD52L2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TPD52L2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11795-1-PBS

Synonyme

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  WB analysis using 11795-1-AP (same clone as 11795-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11795-1-AP (TPD52L2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11795-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 11795-1-AP (same clone as 11795-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11795-1-AP (TPD52L2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11795-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 11795-1-AP (same clone as 11795-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11795-1-AP (TPD52L2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11795-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 11795-1-AP (same clone as 11795-1-PBS)

  IP result of anti-TPD52L2 (IP:11795-1-AP, 3ug; Detection:11795-1-AP 1:800) with HEK-293 cells lysate 2400ug. This data was developed using the same antibody clone with 11795-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 11795-1-AP (same clone as 11795-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11795-1-AP (TPD52L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11795-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 11795-1-AP (same clone as 11795-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11795-1-AP (TPD52L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11795-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 11795-1-AP (same clone as 11795-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11795-1-AP (TPD52L2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11795-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11795-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA TPD52L2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TPD52L2 fusion protein Ag2364
• Vollständiger Name: tumor protein D52-like 2
• Berechnetes Molekulargewicht: 206 aa, 22 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC006804
• Gene symbol: TPD52L2
• Gene ID (NCBI): 7165
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tumor protein D52-like proteins (TPD52) are small coiled-coil motif bearing proteins that were first identified in breast carcinoma. Three human TPD52 members had been identified, named hD52 (TPD52), hD53 (TPD52L1), and hD54 (TPD52L2). The most important characteristic of the protein family is a highly conserved coiled-coil motif that is required for homo- and heteromeric interaction with other TPD52-like proteins. TPD52 and related proteins have been implicated in cell proliferation, apoptosis, and vesicle trafficking. TPD52L2 has five isoforms produced by alternative splicing, and its multiple sites have been identified to be phosphorylated. Interaction of TPD52L2 with MAL2, a novel member of the MAL proteolipid family, may be required for the role of TPD52L2 in vesicle transport.