TPK1 Polyklonaler Antikörper

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TPK1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10942-1-PBS

Synonyme

Thiamine pyrophosphokinase 1, Thiamin pyrophosphokinase 1, PP20, Placental protein 20, hTPK1

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  WB analysis using 10942-1-AP (same clone as 10942-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10942-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 10942-1-AP (same clone as 10942-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10942-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10942-1-AP (same clone as 10942-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10942-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 10942-1-AP (same clone as 10942-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10942-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse small intestine using 10942-1-AP (same clone as 10942-1-PBS)

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10942-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 10942-1-AP (same clone as 10942-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10942-1-PBS in a different storage buffer formulation.

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  WB analysis of human small intestine using 10942-1-AP (same clone as 10942-1-PBS)

  human small intestine tissue were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10942-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 10942-1-AP (same clone as 10942-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10942-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 10942-1-AP (same clone as 10942-1-PBS)

  IP result of anti-TPK1 (IP:10942-1-AP, 4ug; Detection:10942-1-AP 1:500) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 10942-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 10942-1-AP (same clone as 10942-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10942-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 10942-1-AP (same clone as 10942-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10942-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10942-1-AP (same clone as 10942-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10942-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10942-1-AP (same clone as 10942-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10942-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 10942-1-AP (same clone as 10942-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10942-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 10942-1-AP (same clone as 10942-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10942-1-AP (TPK1 Antibody) at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 10942-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 10942-1-AP (same clone as 10942-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10942-1-AP (TPK1 Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 10942-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10942-1-PBS bindet in WB, IHC, IP, Indirect ELISA TPK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TPK1 fusion protein Ag1404
• Vollständiger Name: thiamin pyrophosphokinase 1
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC014552
• Gene symbol: TPK1
• Gene ID (NCBI): 27010
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TPK1(Thiamin pyrophosphokinase 1) is also named as PP20(placental protein 20) and belongs to the thiamine pyrophosphokinase family. It is a cellular enzyme involved in the regulation of thiamine metabolism and catalyzes the conversion of thiamine, a form of vitamin B1, to thiamine pyrophosphate (TDP, or TPP). There is no difference in TPK1 expression in cultured fibroblasts from normal subjects or from patients with thiamine-responsive megaloblastic anemia(PMID:11342111). It can exsit as a dimer(PMID:21552434). Defects in TPK1 are the cause of thiamine metabolism dysfunction syndrome type 5, episodic encephalopathy type (THMD5).