TPM3 Polyklonaler Antikörper

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• KD/KO Validated

TPM3 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 10737-1-AP

Synonyme

Gamma-tropomyosin, Tropomyosin alpha 3 chain, Tropomyosin alpha-3 chain, Tropomyosin-3, Tropomyosin-5

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  WB analysis of mouse skeletal muscle using 10737-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10737-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 10737-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10737-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10737-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10737-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10737-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 10737-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10737-1-AP

  IP result of anti-TPM3 (IP:10737-1-AP, 4ug; Detection:10737-1-AP 1:300) with HeLa cells lysate 2000ug.

• 

  IHC staining of human colon cancer using 10737-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10737-1-AP (TPM3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10737-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10737-1-AP (TPM3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, HEK-293-Zellen, HeLa-Zellen, humanes Herzgewebe, Mausherzgewebe, Rattenherzgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

10737-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA TPM3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TPM3 fusion protein Ag1171
• Vollständiger Name: tropomyosin 3
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC008407
• Gene symbol: TPM3
• Gene ID (NCBI): 7170
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tropomyosins are ubiquitous proteins of 35 to 45 kDa associated with the actin filaments of myofibrils and stress fibers. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins.TPM2 subunit constitutes one part of the TPM dimer, and TPM1 or TPM3 constitutes the other part of the TPM dimer (22064019), causing an extra band of ~70kDa.

Publikationen

Species	Application	Title
human	WB	Bone Res; Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma; Authors - Ming Luo
	IP	Nucleic Acids Res; Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing.; Authors - Ken Asada
human	WB	Oncogene; MTBP suppresses cell migration and filopodia formation by inhibiting ACTN4.; Authors - Agarwal N N
mouse	WB,CoIP	J Am Heart Assoc; Modulation of Abnormal Vasoconstriction Through 2-Hydroxyisobutyrylation of Tropomyosin 3 Lys141: Targeting Histone Deacetylase 3 as a Key Approach; Authors - Pan-Pan Pang
mouse,human	WB	Oncol Res; LncRNA WEE2-AS1 Knockdown Inhibits the Proliferation, Migration and 3 Invasion of Glioma Cells via Regulating miR-29b-2-5p/TPM3 Axis.; Authors - Zhen Jia
	WB	J Proteome Res; Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy; Authors - Yuan Lin