TPMT Polyklonaler Antikörper

TPMT Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 10682-1-AP

Synonyme

Thiopurine S-methyltransferase, EC:2.1.1.67

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  WB analysis using 10682-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10682-1-AP (TPMT antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10682-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10682-1-AP (TPMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 10682-1-AP

  Immunofluorescent analysis of HEK-293 cells using 10682-1-AP (TPMT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

10682-1-AP bindet in WB, IHC, IF/ICC, ELISA TPMT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TPMT fusion protein Ag1074
• Vollständiger Name: thiopurine S-methyltransferase
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28-32 kDa
• GenBank-Zugangsnummer: BC005339
• Gene symbol: TPMT
• Gene ID (NCBI): 7172
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The TPMT gene is localized to chromosome 6p22.3 and is approximately 34 kDa in length. It has an open reading frame (ORF) of 735 bp that encodes for a 245-amino acid peptide. The wild type TPMT allele, expressing the enzyme with normal activity. To date, there have been 30 variant TPMT alleles described (PMID: 22493988). Thiopurine compounds are commonly used in the treatment of childhood acute lymphoblastic leukemia, and as immunosuppressants following organ transplantation or for treatment of various autoimmune disorders. Thiopurine S-methyltransferase (TPMT) is required for detoxification, through S-methylation, of 6-thioguanine nucleotides (TGNs), a byproduct of thiopurine metabolism. Catalog#10682-1-AP is a rabbit polyclonal antibody raised against the full-length of human TPMT. TPMT has a predicted molecular weight of 28 kDa, however, as a result of its phosphorylation it separates on a SDS gel as a band of approximately 28-32 kDa.

Publikationen

Species	Application	Title
human	IHC	Front Cell Dev Biol; Predicting Panel of Metabolism and Immune-Related Genes for the Prognosis of Human Ovarian Cancer.; Authors - Lingyun Zhang
	WB	Metabolites; A Rapid and Reliable Absorbance Assay to Identify Drug-Drug Interactions with Thiopurine Drugs; Authors - Drake A Russell
sheep	WB	PLoS One; TMT-based quantitative proteomics reveals the genetic mechanisms of secondary hair follicle development in fine-wool sheep; Authors - Li-Xia Qiu