TPMT Monoklonaler Antikörper

TPMT Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D4B3

Kat-Nr. : 68251-1-PBS

Synonyme

Thiopurine methyltransferase, thiopurine S methyltransferase, TPMT

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  WB analysis using 68251-1-Ig (same clone as 68251-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68251-1-Ig (TPMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68251-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68251-1-Ig (same clone as 68251-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TPMT antibody (68251-1-Ig, Clone: 1D4B3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68251-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68251-1-PBS bindet in WB, IF/ICC, Indirect ELISA TPMT und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TPMT fusion protein Ag28693
• Vollständiger Name: thiopurine S-methyltransferase
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC005339
• Gene symbol: TPMT
• Gene ID (NCBI): 7172
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The TPMT gene is localized to chromosome 6p22.3 and is approximately 34 kDa in length. It has an open reading frame (ORF) of 735 bp that encodes for a 245-amino acid peptide. The wild type TPMT allele, expressing the enzyme with normal activity. To date, there have been 30 variant TPMT alleles described (PMID: 22493988). Thiopurine compounds are commonly used in the treatment of childhood acute lymphoblastic leukemia, and as immunosuppressants following organ transplantation or for treatment of various autoimmune disorders. Thiopurine S-methyltransferase (TPMT) is required for detoxification, through S-methylation, of 6-thioguanine nucleotides (TGNs), a byproduct of thiopurine metabolism. Catalog#10682-1-AP is a rabbit polyclonal antibody raised against the full-length of human TPMT. TPMT has a predicted molecular weight of 28 kDa, however, as a result of its phosphorylation it separates on a SDS gel as a band of approximately 28-32 kDa.