TPPII Monoklonaler Antikörper

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• KD/KO Validated

TPPII Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

5H6F11

Kat-Nr. : 66017-1-Ig

Synonyme

TPP 2, TPP2, TPPII, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 2, tripeptidyl peptidase II

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 66017-1-Ig

  WB result of TPPII antibody (66017-1-Ig, 1:4000) with si-control and si-TPPII transfected Jurkat cells.

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  WB analysis of HeLa using 66017-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66017-1-Ig (TPPII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66017-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66017-1-Ig (TPPII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 66017-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66017-1-Ig (TPPII antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 66017-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66017-1-Ig(TPPII antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 66017-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66017-1-Ig(TPPII antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 66017-1-Ig

  Immunofluorescent analysis of Hela cells, using TPP2 antibody 66017-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of MCF-7 using 66017-1-Ig

  Immunofluorescent analysis of MCF-7 cells, using TPP2 antibody 66017-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

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  FC experiment of HeLa using 66017-1-Ig

  1X10^6 HeLa cells were stained with 0.2ug TPPII antibody (66017-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

66017-1-Ig bindet in WB, IHC, IF, FC, ELISA TPPII und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TPPII fusion protein Ag5296
• Vollständiger Name: tripeptidyl peptidase II
• Berechnetes Molekulargewicht: 1249 aa, 138 kDa
• Beobachtetes Molekulargewicht: 135 kDa
• GenBank-Zugangsnummer: BC039905
• Gene symbol: TPP2
• Gene ID (NCBI): 7174
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TPPII(Tripeptidyl-peptidase 2) is an evolutionary conserved serine peptidase of the subtilisin family that removes tripeptides from the free NH2 terminus of oligopeptides. The 138-kDa TPP2 polypeptide forms large oligomeric complexes of >1,000 kDa that are detected in the cytoplasm of all cell types. In addition, a membrane associated TPP 2 isoform is present in most cells(PMID:15735022).

Publikationen

Species	Application	Title
human	WB	Nat Commun; Mitochondrial fission induces immunoescape in solid tumors through decreasing MHC-I surface expression.; Authors - Xinyuan Lei
human	WB	J Immunol; Different Antigen-Processing Activities in Dendritic Cells, Macrophages, and Monocytes Lead to Uneven Production of HIV Epitopes and Affect CTL Recognition.; Authors - Jens Dinter