TPPII Monoklonaler Antikörper

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TPPII Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5H6F11

Kat-Nr. : 66017-1-PBS

Synonyme

TPP 2, TPP2, TPPII, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 2, tripeptidyl peptidase II

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  WB analysis of Jurkat using 66017-1-Ig (same clone as 66017-1-PBS)

  WB result of TPPII antibody (66017-1-Ig, 1:4000) with si-control and si-TPPII transfected Jurkat cells. This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66017-1-Ig (same clone as 66017-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66017-1-Ig (TPPII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66017-1-Ig (same clone as 66017-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66017-1-Ig (TPPII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 66017-1-Ig (same clone as 66017-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66017-1-Ig (TPPII antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66017-1-Ig (same clone as 66017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 66017-1-Ig(TPPII antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66017-1-Ig (same clone as 66017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 66017-1-Ig(TPPII antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66017-1-Ig (same clone as 66017-1-PBS)

  Immunofluorescent analysis of Hela cells, using TPP2 antibody 66017-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66017-1-Ig (same clone as 66017-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using TPP2 antibody 66017-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66017-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TPPII und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TPPII fusion protein Ag5296
• Vollständiger Name: tripeptidyl peptidase II
• Berechnetes Molekulargewicht: 1249 aa, 138 kDa
• Beobachtetes Molekulargewicht: 135 kDa
• GenBank-Zugangsnummer: BC039905
• Gene symbol: TPPII
• Gene ID (NCBI): 7174
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TPPII(Tripeptidyl-peptidase 2) is an evolutionary conserved serine peptidase of the subtilisin family that removes tripeptides from the free NH2 terminus of oligopeptides. The 138-kDa TPP2 polypeptide forms large oligomeric complexes of >1,000 kDa that are detected in the cytoplasm of all cell types. In addition, a membrane associated TPP 2 isoform is present in most cells(PMID:15735022).