TPPP Polyklonaler Antikörper

TPPP Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 18742-1-AP

Synonyme

25 kDa brain specific protein, 25 kDa brain-specific protein, EC:3.6.5.-, p24, p25 alpha

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  WB analysis of mouse brain using 18742-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18742-1-AP (TPPP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 18742-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18742-1-AP (TPPP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 18742-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18742-1-AP (TPPP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 18742-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18742-1-AP (TPPP antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of Neuro-2a using 18742-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using TPPP antibody (18742-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Neuro-2a-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

18742-1-AP bindet in WB, IHC, IF/ICC, ELISA TPPP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tubulin polymerization promoting protein
• Berechnetes Molekulargewicht: 219 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24-28 kDa
• GenBank-Zugangsnummer: BC131506
• Gene symbol: TPPP
• Gene ID (NCBI): 11076
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TPPP, also named as TPPP1, P24 and P25, belongs to the TPPP family. TPPP promotes in vitro the polymerization of tubulin into double-walled tubules and polymorphic aggregates or bundled stabilized microtubules blocks. When overexpressed, TPPP inhibits mitotic spindle assembly and nuclear envelope breakdown, apparently without affecting other cellular events.

Publikationen

Species	Application	Title
mouse	IF	Cell; The Golgi Outpost Protein TPPP Nucleates Microtubules and Is Critical for Myelination.; Authors - Meng-Meng Fu
human	WB,IHC,IF	FASEB J; EBV-miR-BART12 accelerates migration and invasion in EBV-associated cancer cells by targeting tubulin polymerization-promoting protein 1.; Authors - Yingfen Wu
human	WB	Stem Cells Int; Enhanced Effect of IL-1β-Activated Adipose-Derived MSCs (ADMSCs) on Repair of Intestinal Ischemia-Reperfusion Injury via COX-2-PGE2 Signaling.; Authors - Liu Liu
	WB	ACS Infect Dis; Repositioning Brusatol as a Transmission Blocker of Malaria Parasites; Authors - Amelia Cox
rat	WB	J Mol Cell Cardiol; Defective autophagy triggered by arterial cyclic stretch promotes neointimal hyperplasia in vein grafts via the p62/nrf2/slc7a11 signaling pathway; Authors - Yi Chen