TPPP Polyklonaler Antikörper

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• KD/KO Validated

TPPP Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25040-1-AP

Synonyme

25 kDa brain specific protein, 25 kDa brain-specific protein, EC:3.6.5.-, p24, p25 alpha

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  WB analysis of MCF-7 using 25040-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25040-1-AP (TPPP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 25040-1-AP

  WB result of TPPP antibody (25040-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPPP transfected SH-SY5Y cells.

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  WB analysis using 25040-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25040-1-AP (TPPP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25040-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25040-1-AP (TPPP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 25040-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25040-1-AP (TPPP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 25040-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25040-1-AP (TPPP Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 25040-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25040-1-AP (TPPP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 25040-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25040-1-AP (TPPP Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 25040-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TPPP antibody (25040-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, MCF-7-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25040-1-AP bindet in WB, IHC, IF/ICC, ELISA TPPP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TPPP fusion protein Ag19159
• Vollständiger Name: tubulin polymerization promoting protein
• Berechnetes Molekulargewicht: 219 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24-29 kDa
• GenBank-Zugangsnummer: BC131506
• Gene symbol: TPPP
• Gene ID (NCBI): 11076
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.