TPPP Monoklonaler Antikörper

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• KD/KO Validated

TPPP Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, Huhn, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E5F5

Kat-Nr. : 68048-1-PBS

Synonyme

p25 alpha, p24, EC:3.6.5.-, 25 kDa brain-specific protein, 25 kDa brain specific protein

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  WB analysis using 68048-1-Ig (same clone as 68048-1-PBS)

  Brain tissues were subjected to SDS PAGE followed by western blot with 68048-1-Ig (TPPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68048-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 68048-1-Ig (same clone as 68048-1-PBS)

  WB result of TPPP antibody (68048-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPPP transfected SH-SY5Y cells. This data was developed using the same antibody clone with 68048-1-PBS in a different storage buffer formulation.

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  WB analysis of pig cerebellum using 68048-1-Ig (same clone as 68048-1-PBS)

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 68048-1-Ig (TPPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68048-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit cerebellum using 68048-1-Ig (same clone as 68048-1-PBS)

  rabbit cerebellum tissue were subjected to SDS PAGE followed by western blot with 68048-1-Ig (TPPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68048-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat cerebellum using 68048-1-Ig (same clone as 68048-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 68048-1-Ig (TPPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68048-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 68048-1-Ig (same clone as 68048-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 68048-1-Ig (TPPP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68048-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 68048-1-Ig (same clone as 68048-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 68048-1-Ig (TPPP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68048-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68048-1-Ig (same clone as 68048-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68048-1-Ig (TPPP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68048-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68048-1-Ig (same clone as 68048-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68048-1-Ig (TPPP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68048-1-PBS in a different storage buffer formulation.

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  IF Staining of Neuro-2a using 68048-1-Ig (same clone as 68048-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using TPPP antibody (68048-1-Ig, Clone: 1E5F5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68048-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68048-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TPPP und zeigt Reaktivität mit Hausschwein, Huhn, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, Huhn, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TPPP fusion protein Ag19046
• Vollständiger Name: tubulin polymerization promoting protein
• Berechnetes Molekulargewicht: 219 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24-29 kDa
• GenBank-Zugangsnummer: BC131506
• Gene symbol: TPPP
• Gene ID (NCBI): 11076
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TPPP, also named as TPPP1, P24 and P25, belongs to the TPPP family. TPPP promotes in vitro the polymerization of tubulin into double-walled tubules and polymorphic aggregates or bundled stabilized microtubules blocks. When overexpressed, TPPP inhibits mitotic spindle assembly and nuclear envelope breakdown, apparently without affecting other cellular events.