TPPP3 Polyklonaler Antikörper

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TPPP3 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15057-1-PBS

Synonyme

Tubulin polymerization-promoting protein family member 3, TPPP/p20, p25gamma, p20, CGI-38

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  WB analysis of HEK-293 cells using 15057-1-AP (same clone as 15057-1-PBS)

  WB result of anti-TPPP3 (15057-1-AP, 1:500) with HEK-293 cells (siRNA transfected with) by Dr. Yupeng Chen. (Negative control or TPPP3 specific siRNAs were transfected into HEK-293 cells with the final concentration of 20 nM. Forty eight hours after transfection, cells were lysed with RIPA buffer and subjected to SDS PAGE). This data was developed using the same antibody clone with 15057-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 15057-1-AP (same clone as 15057-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15057-1-AP (TPPP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15057-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 15057-1-AP (same clone as 15057-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15057-1-AP (TPPP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15057-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 15057-1-AP (same clone as 15057-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15057-1-AP (TPPP3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15057-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 15057-1-AP (same clone as 15057-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15057-1-AP (TPPP3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15057-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15057-1-PBS bindet in WB, IHC, Indirect ELISA TPPP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TPPP3 fusion protein Ag7040
• Vollständiger Name: tubulin polymerization-promoting protein family member 3
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 19-20 kDa
• GenBank-Zugangsnummer: BC000691
• Gene symbol: TPPP3
• Gene ID (NCBI): 51673
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TPPP3 (tubulin polymerization promoting protein 3), also named as TPPP/p20, is a new member of the TPPP family which binds tubulin and induces tubulin polymerization and microtubule bundling.TPPP3 has been shown to play an important role in cell proliferation and may be implicated in tumorigenesis and metastasis (19633818). TPPP3 was also identified as specific marker for the connective tissues that envelope tendons due to its distinct expression in the developing tendon sheath (19235716). This antibody detects the 19-20 kDa TPPP3 in HeLa and PC-3 cells. The non-specific bands between 40 and 60 kDa were also occasionally observed.