TPR Rekombinanter Antikörper

TPR Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

230413A2

Kat-Nr. : 83177-5-PBS

Synonyme

Nucleoprotein TPR, 230413A2

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  WB analysis using 83177-5-RR (same clone as 83177-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83177-5-RR (TPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83177-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 83177-5-RR (same clone as 83177-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83177-5-RR (TPR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83177-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83177-5-RR (same clone as 83177-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TPR antibody (83177-5-RR, Clone: 230413A2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83177-5-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

83177-5-PBS bindet in WB, IHC, IF/ICC, ELISA TPR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: translocated promoter region (to activated MET oncogene)
• Berechnetes Molekulargewicht: 267 kDa
• Beobachtetes Molekulargewicht: ~270 kDa
• GenBank-Zugangsnummer: NM_003292
• Gene symbol: TPR
• Gene ID (NCBI): 7175
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nucleoporin TPR was originally identified as the oncogenic activator of the met, raf, and trk protooncogenes. TPR is a large 270 kDa protein with a bipartite structure consisting of a ~1600-residue a-helical coiled-coil N-terminal domain and a highly acidic non-coiled ~800 amino acid carboxy terminus (PMID: 22253824). TPR is localized exclusively to intranuclear filaments associated with the nucleoplasmic side of the NPC, by directly binding to Nup153 (PMID: 12802065).