TPRG1L Rekombinanter Antikörper

TPRG1L Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241070G6

Kat-Nr. : 83989-4-RR

Synonyme

241070G6, FAM79A, h mover, Mossy fiber terminal-associated vertebrate-specific presynaptic protein, MOVER

---

• 

  WB analysis using 83989-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83989-4-RR (TPRG1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 83989-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83989-4-RR (TPRG1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 83989-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83989-4-RR (TPRG1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 83989-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83989-4-RR against Human TPRG1L were performed. The affinity constant is 0.234 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83989-4-RR bindet in WB, IHC, ELISA TPRG1L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TPRG1L fusion protein Ag22585
• Vollständiger Name: tumor protein p63 regulated 1-like
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC019034
• Gene symbol: TPRG1L
• Gene ID (NCBI): 127262
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TPRG1L (Tumor protein p63-regulated gene 1-like protein) belongs to the presynaptic protein involved in the synaptic transmission tuning. It can regulate synaptic release probability by decreasing the calcium sensitivity of release. TPRG1L has two isoforms with molecular weights of 30kd and 23kd respectively. This antibody can recognize the 30kd isoform and may also recognize the 23kd isoform.