TPX2 Monoklonaler Antikörper

TPX2 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2E10C1

Kat-Nr. : 68712-1-Ig

Synonyme

2E10C1, C20orf1, C20orf2, Differentially expressed in cancerous and non-cancerous lung cells 2, DIL 2

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  WB analysis using 68712-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68712-1-Ig (TPX2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68712-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68712-1-Ig (TPX2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 68712-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 68712-1-Ig (TPX2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 68712-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68712-1-Ig (TPX2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 68712-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using TPX2 antibody (68712-1-Ig, Clone: 2E10C1 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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  IF Staining of U2OS using 68712-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TPX2 antibody (68712-1-Ig, Clone: 2E10C1 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen, A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68712-1-Ig bindet in WB, IF/ICC, ELISA TPX2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TPX2 fusion protein Ag33683
• Vollständiger Name: TPX2, microtubule-associated, homolog (Xenopus laevis)
• Berechnetes Molekulargewicht: 747 aa, 86 kDa
• Beobachtetes Molekulargewicht: 98 kDa
• GenBank-Zugangsnummer: BC020207
• Gene symbol: TPX2
• Gene ID (NCBI): 22974
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TPX2, also known as DIL2, p10, is a microtubule organization- and cell cycle-associated protein. In the early stage of mitosis, TPX2 is released in a RanGTP-dependent manner and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. Furthermore, during interphase, TPX2 is involved in DNA damage response by regulation of γ-H2AX signals(PMID: 25914189). Aberrant expression of TPX2 leads to improper spindle assembly and chromosomal instability. Overexpressed in cancers, TPX2 is being established as the marker for the diagnosis and prognosis of malignancies(PMID: 24556998).