TRADD Polyklonaler Antikörper

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• KD/KO Validated

TRADD Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 15468-1-AP

Synonyme

CD120a, TNFR1-associated DEATH domain protein, TNFRSF1A, TNFRSF1A-associated via death domain, Tumor necrosis factor receptor type 1-associated DEATH domain protein

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  WB analysis using 15468-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15468-1-AP (TRADD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15468-1-AP

  WB result of TRADD antibody (15468-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRADD transfected A549 cells.

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  WB analysis using 15468-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15468-1-AP (TRADD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 15468-1-AP

  IP result of anti-TRADD (IP:15468-1-AP, 4ug; Detection:15468-1-AP 1:500) with Jurkat cells lysate 4000 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HL-60-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche IP: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

15468-1-AP bindet in WB, IHC, IF, IP, ELISA TRADD und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRADD fusion protein Ag7768
• Vollständiger Name: TNFRSF1A-associated via death domain
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC004491
• Gene symbol: TRADD
• Gene ID (NCBI): 8717
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Res; The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.; Authors - Jia-Yuan Zhang
human	WB	Free Radic Biol Med; TRAF2 inhibits senescence in hepatocellular carcinoma cells via regulating the ROMO1/ NAD+/SIRT3/SOD2 axis; Authors - Jiping Yao
mouse	WB,IP,IF	Free Radic Biol Med; Kv7.4 channel is a key regulator of vascular inflammation and remodeling in neointimal hyperplasia and abdominal aortic aneurysms.; Authors - Xi-Zhenzi Fan
mouse	WB	Int J Biol Sci; Mannan-Binding Lectin Deficiency Limits Inflammation-induced Myeloid-Derived Suppressor Cells Expansion via Modulating Tumor Necrosis Factor Alpha-triggered Apoptosis.; Authors - Lijun Dong
mouse	WB	Mol Med; Knockdown of circROBO2 attenuates acute myocardial infarction through regulating the miR-1184/TRADD axis.; Authors - Tian-Ping Chen
mouse	WB	Arch Dermatol Res; PeiTuQingXin formula alleviated atopic dermatitis symptoms via inhibiting TRADD/TRAF2/RIP1 complex mediated NF-κB signaling pathway activation; Authors - Zixuan Cheng