TRAF3 Polyklonaler Antikörper

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• KD/KO Validated

TRAF3 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 18099-1-AP

Synonyme

CAP 1, CAP1, CD40 binding protein, CD40bp, CRAF1, LAP1, LMP1 associated protein 1, TRAF3

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Galerie der Validierungsdaten

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  WB analysis using 18099-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18099-1-AP (TRAF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 18099-1-AP

  WB result of TRAF3 antibody (18099-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAF3 transfected HEK-293 cells.

• 

  WB analysis using 18099-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18099-1-AP (TRAF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of multi-cells using 18099-1-AP

  WB results of 18099-1-aP (anti TRAF3).

• 

  WB analysis of HEK-293 using 18099-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18099-1-AP (TRAF3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 18099-1-AP

  IP result of anti-TRAF3 (IP:18099-1-AP, 4ug; Detection:18099-1-AP 1:1000) with Raji cells lysate 3160 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HEK-293-Zellen, HeLa-Zellen, Multizellen, NIH/3T3-Zellen, Raji-Zellen
• Erfolgreiche IP: Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 17 publications below
• IF: See 1 publications below

Produktinformation

18099-1-AP bindet in WB, IP, IF, ELISA TRAF3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRAF3 fusion protein Ag12185
• Vollständiger Name: TNF receptor-associated factor 3
• Berechnetes Molekulargewicht: 568 aa, 64 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC075087
• Gene symbol: TRAF3
• Gene ID (NCBI): 7187
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNF receptor-associated factor 3 (TRAF3) has many alternative names including CAP-1, CD40 receptor-associated factor 1(CRAF1), CD40-binding protein(CD40BP) and LMP1-associated protein 1 (LAP1). TRAF3 regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of innate immune response. It modulates signaling pathways leading to the production of cytokines and interferon. As an essential constituent of several E3 ubiquitin-protein ligase complexes, TRAF3 promote 'Lys-63'-linked ubiquitination of target proteins. Moreover, TRAF3 undergoes both 'Lys-48'-linked and 'Lys-63'-linked ubiquitination in response to various stimuli and is deubiquitinated by OTUB1, OTUB2 and OTUD5. Several alternatively spliced transcript variants encoding distinct isoforms (64 kDa and 55 kDa) have been reported.

Publikationen

Species	Application	Title
human	WB	Signal Transduct Target Ther; TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion; Authors - Wenwen Zheng ; KD Validated
mouse	WB	J Exp Med; TRIM24 facilitates antiviral immunity through mediating K63-linked TRAF3 ubiquitination.; Authors - Qingchen Zhu
pig	WB	Nucleic Acids Res; The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response.; Authors - Xin Zong
human	WB	EMBO J; Dimer-specific immunoprecipitation of active caspase-2 identifies TRAF proteins as novel activators.; Authors - Alexander C Robeson ; KD Validated
human	WB	Front Immunol; The C-Terminal Effector Domain of Non-Structural Protein 1 of Influenza A Virus Blocks IFN-β Production by Targeting TNF Receptor-Associated Factor 3.; Authors - Wei Qian
human	WB	Cancer Lett; Up-regulation of OLR1 expression by TBC1D3 through activation of TNFα/NF-κB pathway promotes the migration of human breast cancer cells.; Authors - Bei Wang

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