TRAF3IP3 Polyklonaler Antikörper

TRAF3IP3 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18110-1-PBS

Synonyme

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  WB analysis of Jurkat using 18110-1-AP (same clone as 18110-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18110-1-AP (TRAF3IP3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18110-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 18110-1-AP (same clone as 18110-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18110-1-AP (TRAF3IP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 18110-1-AP (same clone as 18110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18110-1-AP (TRAF3IP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18110-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 18110-1-AP (same clone as 18110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18110-1-AP (TRAF3IP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 18110-1-AP (same clone as 18110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18110-1-AP (TRAF3IP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18110-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18110-1-PBS bindet in WB, IHC, Indirect ELISA TRAF3IP3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRAF3IP3 fusion protein Ag12559
• Vollständiger Name: TRAF3 interacting protein 3
• Berechnetes Molekulargewicht: 353aa,41 kDa; 551aa,64 kDa
• Beobachtetes Molekulargewicht: 64 kDa, 47 kDa, 41 kDa
• GenBank-Zugangsnummer: BC110302
• Gene symbol: TRAF3IP3
• Gene ID (NCBI): 80342
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRAF3 interacting protein 3 (TRAF3IP3) is an essential adapter protein containing a coiled-coil domain that affects both innate and adaptive immunity. It plays a crucial role in the regulation of thymocyte development (PMID:26195727), and it also regulates T-cell stability and function by recruiting the serine-threonine phosphatase catalytic subunit (PPP2CA) to the lysosome, thereby facilitating the interaction of PP2Ac with the mTORC1 component RPTOR and restricting glycolytic metabolism (PMID:30115741). TRAF3IP3 responses to viral infection, facilitates the recruitment of TRAF3 to MAVS within mitochondria leading to IRF3 activation and interferon production (PMID:31390091). TRAF3IP3 has 3 isoforms produced by alternative splicing with the molecular weight of 64 kDa, 47 kDa and 41 kDa.