TRAF4 Monoklonaler Antikörper

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• KD/KO Validated

TRAF4 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

2C9A11

Kat-Nr. : 66755-1-Ig

Synonyme

CART1, MLN 62, MLN62, RING finger protein 83, RNF83, TRAF4

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Galerie der Validierungsdaten

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  WB analysis using 66755-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66755-1-Ig (TRAF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 66755-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66755-1-Ig (TRAF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 66755-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66755-1-Ig (TRAF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 66755-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66755-1-Ig (TRAF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human urothelial carcinoma using 66755-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 66755-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66755-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66755-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66755-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66755-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66755-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66755-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66755-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66755-1-Ig (TRAF4 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Daudi-Zellen, Hausschwein-Hirngewebe, Hausschwein-Milzgewebe, HEK-293-Zellen, HeLa-Zellen, humanes Hirngewebe, NCCIT-Zellen, Raji-Zellen, Ramos-Zellen, THP-1-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Urothelkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

66755-1-Ig bindet in WB, IHC, IF, ELISA TRAF4 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TRAF4 fusion protein Ag27221
• Vollständiger Name: TNF receptor-associated factor 4
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 50-54 kDa
• GenBank-Zugangsnummer: BC001769
• Gene symbol: TRAF4
• Gene ID (NCBI): 9618
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The tumor necrosis factor receptor-associated factor (TRAF) family, consisting of seven members (TRAF1-7), is a group of signaling adaptors which participate in various signaling pathways through binding of the tumor necrosis factor receptor superfamily. As one of the important member of TRAF family proteins, TRAF4 was initially isolated from breast carcinomas and identified as the first member of the TRAF family to be up-regulated in human carcinomas. TRAF4 is required during embryogenesis in key biological processes including the formation of the trachea, the development of the axial skeleton, and the closure of the neural tube. TRAF4 deficiency leads to severe developmental alterations.

Publikationen

Species	Application	Title
human	WB,IF	Int J Mol Sci; TRAF4 Promotes the Proliferation of Glioblastoma by Stabilizing SETDB1 to Activate the AKT Pathway; Authors - Hongyu Gu ; KD Validated
mouse	WB	Anticancer Drugs; High expression of TRAF4 predicts poor prognosis in tamoxifen-treated breast cancer and promotes tamoxifen resistance.; Authors - Jun Zhou ; KD Validated