TRAF5 Polyklonaler Antikörper

TRAF5 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26917-1-AP

Synonyme

RING finger protein 84, RNF84, TRAF5

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 26917-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26917-1-AP (TRAF5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

26917-1-AP bindet in WB,ELISA TRAF5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRAF5 fusion protein Ag25284
• Vollständiger Name: TNF receptor-associated factor 5
• Berechnetes Molekulargewicht: 557 aa, 64 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC029600
• Gene symbol: TRAF5
• Gene ID (NCBI): 7188
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRAF5 (also known as TNF receptor-associated factor 5, Ring finger protein 84, RNF8, MGC:39780) is a member of the tumor necrosis factor receptor-associated (TRAF) family of proteins that are characterized by the presence of a meprin and TRAF homology (MATH) domain, a RING-type zinc finger domain, and two TRAF-type zinc finger domains (https://www.uniprot.org/uniprot/O00463). TRAF5 is a scaffold protein that associates with and mediates signal transduction by cell surface receptors belonging to the tumor necrosis factor (TNF) superfamily, including CD27, CD30, CD40, and lymphotoxin-β receptor, positively regulating activation of the canonical NF-κB pathway and c-Jun NH2-terminal kinase (JNK) activation by these receptors (PMIDs: 8999898, 9582383, 8790348, 8663299).

The RING-type zinc finger domain of TRAF5 exhibits ubiquitin protein ligase activity and is responsible for Lys-63-linked polyubiquitination of protein targets (PMIDs:23758787, 29176576). This domain has been demonstrated to be required for TRAF5-mediated activation of NF-κB (PMID:8663299).

What is the molecular weight of TRAF5?

TRAF5 is a 557 amino acid protein that has a predicted molecular weight of 64 kDa.

What is the subcellular localization of TRAF-5?

TRAF5 is a cytoplasmic protein (PMID:11607847).

What is the tissue specificity of TRAF5?

TRAF5 is broadly expressed in the spleen, thymus, prostate, testis, ovary, small intestine, colon, and peripheral blood. The highest level of expression is observed in Peyer's patches in the small intestine (https://www.uniprot.org/uniprot/O00463).

What is the role of TRAF5 and immune cell function?

Studies in traf5-/- mice have shown that TRAF5 plays a key role in CD40-mediated signaling in B-cells and CD27-mediated signaling in T-cells. In B-cells, deletion of the traf5 gene results in defects in cell proliferation and upregulation of various cell surface molecules, including CD23, CD54, CD80, CD86, and Fas, in response to CD40 stimulation. Similarly, upregulation of CD27-mediated costimulatory signals is also impaired in traf5−/− T cells. These effects lead to impairments in lymphocyte activation in vivo (PMID:10449775).

TRAF5 also plays a critical role in in vitro signaling and in vivo function in B-cells by the Epstein-Barr Virus (EBV)-encoded oncogenic mimic of CD40, latent membrane protein 1 (LMP1), which is encoded by the virus and is essential for transformation of B lymphocytes (PMID:19805155).